{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:44:27Z","timestamp":1767962667349,"version":"3.49.0"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,7,20]],"date-time":"2014-07-20T00:00:00Z","timestamp":1405814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of University and Research","award":["FAR 2010-2013"],"award-info":[{"award-number":["FAR 2010-2013"]}]},{"name":"Rete nazionale per lo studio del proteoma umano","award":["RBRN07BMCT_11"],"award-info":[{"award-number":["RBRN07BMCT_11"]}]},{"name":"i-MODE-CKD","award":["FP7-PEOPLE-2013-ITN"],"award-info":[{"award-number":["FP7-PEOPLE-2013-ITN"]}]},{"name":"Healthcare Research","award":["BM1104"],"award-info":[{"award-number":["BM1104"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Matrix\u2010assisted laser desorption\/ionization (<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>) imaging mass spectrometry (<jats:styled-content style=\"fixed-case\">IMS<\/jats:styled-content>) is a unique proteomic technology that explores the spatial distribution of biomolecules directly <jats:italic>in situ<\/jats:italic>, thus integrating molecular and morphological information. The possibility of correlating distribution maps of multiple analyses with cytological features makes it an ideal research tool for discovering new diagnostic markers. A previous study showed that <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IMS<\/jats:styled-content> could help discrimination between different types of thyroid lesions, especially papillary thyroid carcinoma (<jats:styled-content style=\"fixed-case\">PTC<\/jats:styled-content>); the present feasibility study on <jats:italic>ex vivo<\/jats:italic> fine needle aspiration (<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>) smears describes its potential in detecting new proteomic targets of other thyroid lesions (follicular lesions, medullary carcinoma).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IMS<\/jats:styled-content> was conducted on <jats:italic>ex vivo <\/jats:italic><jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>s obtained from surgical specimens and corresponding <jats:italic>in vivo<\/jats:italic> samples. Differences between proteomic profiles of different thyroid lesions were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparing the protein profiles of hyperplastic nodules obtained from three different patients with each other, and with a new <jats:styled-content style=\"fixed-case\">PTC<\/jats:styled-content>, showed a high degree of concordance, indicating good reproducibility of the <jats:styled-content style=\"fixed-case\">IMS<\/jats:styled-content> technology on cytological samples, suggesting its potential as a tool for biomarker discovery. Furthermore, comparison of the average proteomic profiles of hyperplastic nodules with a H\u00fcrthle cell adenoma revealed significant differences, underlying the capability of <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IMS<\/jats:styled-content> to distinguish between different thyroid lesions. Finally, the proteomic profile of medullary thyroid carcinoma was also characterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results confirmed the possible role of <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IMS<\/jats:styled-content> in the search for diagnostic targets of <jats:styled-content style=\"fixed-case\">PTC<\/jats:styled-content> and follicular lesions, which could be applied in larger trials aimed at the identification of proteins, convertible to cost\u2010effective diagnostic tools such as immunohistochemistry. These tests could be used to analyse <jats:italic>in vivo<\/jats:italic> cytological smears, improving the preoperative diagnosis of indeterminate thyroid nodules.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12166","type":"journal-article","created":{"date-parts":[[2014,7,21]],"date-time":"2014-07-21T04:11:18Z","timestamp":1405915878000},"page":"318-324","source":"Crossref","is-referenced-by-count":30,"title":["Proteomics for the diagnosis of thyroid lesions: preliminary report"],"prefix":"10.1111","volume":"26","author":[{"given":"F.","family":"Pagni","sequence":"first","affiliation":[{"name":"Department of Pathology San Gerardo Hospital University Milan Bicocca Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Mainini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Proteomics University Milan Bicocca Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Garancini","sequence":"additional","affiliation":[{"name":"Department of Surgery San Gerardo Hospital Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Bono","sequence":"additional","affiliation":[{"name":"Department of Pathology San Gerardo Hospital University Milan Bicocca Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Vanzati","sequence":"additional","affiliation":[{"name":"Department of Pathology San Gerardo Hospital University Milan Bicocca Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Giardini","sequence":"additional","affiliation":[{"name":"Department of Surgery San Gerardo Hospital Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Scardilli","sequence":"additional","affiliation":[{"name":"Department of Surgery San Gerardo Hospital Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Goffredo","sequence":"additional","affiliation":[{"name":"Department of Surgery San Gerardo Hospital Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Proteomics University Milan Bicocca Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Galli","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Proteomics University Milan Bicocca Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"De Sio","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Proteomics University Milan Bicocca Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Magni","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Proteomics University Milan Bicocca Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,20]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12085"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21566"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2009.0110"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-1469"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203208"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20063"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.06.015"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-013-9273-8"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072911"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2006.16.1131"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr8000404"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12093"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12166","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T13:56:38Z","timestamp":1694440598000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,20]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/cyt.12166"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12166","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,20]]}}}