{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:46:03Z","timestamp":1772120763632,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,8,13]],"date-time":"2014-08-13T00:00:00Z","timestamp":1407888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The correct identification of fungal organisms is important for the appropriate clinical management of patients. It becomes difficult in necrotic smears when the tissue response is not clearly discernible. It is difficult to distinguish between <jats:italic>histoplasma<\/jats:italic> and <jats:italic>cryptococcus<\/jats:italic> in severely necrotic cases, where both appear as variably sized clear refractile haloes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four cases of adrenal necrotic <jats:italic>histoplasma<\/jats:italic> infection were studied and the morphology was compared with that of non\u2010necrotic histoplasmosis and cases of cryptococcal infection. Eleven cases were analysed in fine needle aspiration cytology (<jats:styled-content style=\"fixed-case\">FNAC<\/jats:styled-content>) smears. Ziehl\u2013Neelsen (<jats:styled-content style=\"fixed-case\">ZN<\/jats:styled-content>) stain was performed to exclude tuberculosis in necrotic smears. A clinical and serology correlation was performed where available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Necrotic cases of <jats:italic>histoplasma<\/jats:italic> infection revealed negative refractile clear haloes similar to those of <jats:italic>cryptococcus<\/jats:italic>. <jats:italic>Histoplasma<\/jats:italic> showed methylene blue\u2010stained organisms in <jats:styled-content style=\"fixed-case\">ZN<\/jats:styled-content> stains, whereas the <jats:italic>cryptococcus<\/jats:italic> cases were negative. Similar methylene blue\u2010stained organisms were seen in non\u2010necrotic <jats:italic>histoplasma<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>As a result of morphological overlap between <jats:italic>cryptococcus<\/jats:italic> and <jats:italic>histoplasma<\/jats:italic>, the distinction between the two fungi can be difficult in many cases. <jats:styled-content style=\"fixed-case\">ZN<\/jats:styled-content> staining appears to have a role in the differentiation of these fungi in severely necrotic cases. This observation needs to be validated on a larger number of cases with complete correlation with clinical, serology and treatment records.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12180","type":"journal-article","created":{"date-parts":[[2014,8,14]],"date-time":"2014-08-14T01:44:52Z","timestamp":1407980692000},"page":"244-249","source":"Crossref","is-referenced-by-count":8,"title":["Differentiation of <i>histoplasma<\/i> and <i>cryptococcus<\/i> in cytology smears: a diagnostic dilemma in severely necrotic cases"],"prefix":"10.1111","volume":"26","author":[{"given":"R.","family":"Ranjan","sequence":"first","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. K.","family":"Iyer","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Mathur","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,8,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00053-10"},{"key":"e_1_2_6_3_1","first-page":"235","volume-title":"Theory and Practice of Histotechnology","author":"Sheehan D","year":"1980"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652009000500004"},{"key":"e_1_2_6_5_1","first-page":"1007","volume-title":"Pathology of Infectious Diseases","author":"Chandler FW","year":"1997"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.9.2519-2522.1993"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2009.02.006"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/648421"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2009.00693.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(89)90453-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2840070123"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1311.070665"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12180","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T23:59:37Z","timestamp":1695513577000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,13]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/cyt.12180"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12180","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,13]]}}}