{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:20:42Z","timestamp":1765887642975},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T00:00:00Z","timestamp":1412208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Office of the NHS Cancer Screening Programmes"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The use of testing for human papillomavirus (HPV) is now recognized as an efficient means of triaging women with low\u2010grade cytological abnormalities to either immediate referral to colposcopy or return to routine recall. We aimed to determine the sensitivity and specificity of each of four newer tests for HPV relative to the Qiagen Hybrid Capture 2 (HC2) assay in order to determine whether they could be approved for use in triage in the NHS cervical screening programme.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared the performance of each of four different HPV assays (Abbott M2000, Roche Cobas, Hologic Cervista and Gen\u2010Probe APTIMA) with that of HC2 in order to determine the sensitivity and specificity of each test relative to HC2 for the detection of cervical intraepithelial neoplasia (CIN) grade 2 or worse, using routine cytology samples reported as borderline (atypical squamous cells) or mild dyskaryosis (low\u2010grade squamous intraepithelial lesion) from six laboratories in England. All women who were found to be HPV positive on any test were referred to colposcopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Between 2072 and 4217 tests were performed with each assay. All four assays were shown to have a relative sensitivity of no worse than 95% compared with HC2 when a cut\u2010off of 2 relative light units (RLU) was used. All assays had higher relative specificity than HC2 for both borderline and mild cytology referrals (1.06\u20131.61).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>All assays tested met the criteria required. Consequently, all have now been approved for use in HPV triage in the NHS cervical screening programme.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12210","type":"journal-article","created":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T08:09:12Z","timestamp":1412237352000},"page":"373-380","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of the performance of <scp>HPV<\/scp> tests in women with abnormal cytology: results of a study within the <scp>NHS<\/scp> cervical screening programme"],"prefix":"10.1111","volume":"26","author":[{"given":"S. M.","family":"Moss","sequence":"first","affiliation":[{"name":"Centre for Cancer Prevention Queen Mary University of London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Cubie","sequence":"additional","affiliation":[{"name":"Scottish HPV Reference Laboratory Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Denton","sequence":"additional","affiliation":[{"name":"North Bristol NHS Trust Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sargent","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Muir","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Public Health England Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. B.","family":"Vipond","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Public Health England Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Winder","sequence":"additional","affiliation":[{"name":"NHS Cancer Screening Programmes Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kitchener","sequence":"additional","affiliation":[{"name":"Institute of Cancer Sciences University of Manchester Manchester Academic Health Centre UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.38701.440961.7C"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.38698.458866.7C"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.10.007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.326"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.435"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0b013e31829c97d8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2013.11.001"},{"key":"e_1_2_7_9_1","volume-title":"Achievable Standards, Benchmarks for Reporting and Criteria for Evaluating Cervical Cytopathology","author":"National Health Service Cervical Screening Programme","year":"2013"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00896-09"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.22"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2013.01.018"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00181-12"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02311-10"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25726"},{"key":"e_1_2_7_17_1","first-page":"43","article-title":"The Abbott RealTime High Risk HPV test is a clinically validated human papillomavirus assay for triage in the referral population and use in primary cervical cancer screening in women 30\u00a0years and older: a review of validation studies","volume":"22","author":"Poljak M","year":"2013","journal-title":"Acta Dermatovenerol Alp Panonica Adriat"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05552-11"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.104"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12083"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T13:52:34Z","timestamp":1694440354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,2]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/cyt.12210"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12210","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,2]]}}}