{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T21:50:56Z","timestamp":1775339456459,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,2,14]],"date-time":"2015-02-14T00:00:00Z","timestamp":1423872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007638","name":"The Kidney Foundation, Japan","doi-asserted-by":"crossref","award":["JKF 12-3"],"award-info":[{"award-number":["JKF 12-3"]}],"id":[{"id":"10.13039\/501100007638","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Damage and detachment of podocytes and loss into the urine have been implicated in the progression of kidney diseases. The purpose of this study was to investigate the potential role of urine cytology based on SurePath<jats:sup>\u2122<\/jats:sup> combined with immunoenzyme staining using Wilms\u2019 tumour 1 (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1) antibody as a podocyte marker in the discrimination of normality and non\u2010renal urinary tract disease from kidney disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010six patients with kidney disease, 45 patients with lower urinary tract disease and 30 healthy volunteers were examined. Urine cytology slides were prepared using the SurePath method and immunoenzyme stained with <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1 antibody, and the number of <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1\u2010positive cells was counted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In kidney disease, <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1\u2010positive cells were found in 33 (50%) of 66 samples. No <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1\u2010positive cells were found in 45 patients with lower urinary tract disease or in 30 healthy volunteers. The positive rates for <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1 varied with disease type, but not significantly: immunoglobulin A (IgA) nephropathy, (14\/23); membranous glomerulonephritis, (4\/10); Henoch\u2013Sch\u00f6nlein purpura nephritis, (3\/5); diabetic glomerulopathy, (5\/5); minor glomerular abnormality\/minimal change nephrotic syndrome (0\/4).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results suggest that <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1 immunoenzyme staining of urine cytology can be used to detect some types of kidney disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12227","type":"journal-article","created":{"date-parts":[[2015,2,14]],"date-time":"2015-02-14T10:13:14Z","timestamp":1423908794000},"page":"43-49","source":"Crossref","is-referenced-by-count":11,"title":["<scp>WT<\/scp>1 immunoenzyme staining using SurePath<sup>\u2122<\/sup> processed urine cytology helps to detect kidney disease"],"prefix":"10.1111","volume":"27","author":[{"given":"H.","family":"Ohsaki","sequence":"first","affiliation":[{"name":"Department of Medical Technology Ehime Prefectural University of Health Sciences Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Sofue","sequence":"additional","affiliation":[{"name":"Department of Cardiorenal and Cerebrovascular Medicine Faculty of Medicine Kagawa University Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Department of Cardiorenal and Cerebrovascular Medicine Faculty of Medicine Kagawa University Kagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Nishijima","sequence":"additional","affiliation":[{"name":"Department of Cardiorenal and Cerebrovascular Medicine Faculty of Medicine Kagawa University Kagawa 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