{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:57:40Z","timestamp":1760299060852},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,28]],"date-time":"2014-12-28T00:00:00Z","timestamp":1419724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Our aim was to evaluate the feasibility and diagnostic accuracy of liquid\u2010based cytology (<jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>) on lymph node fine needle aspiration (<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> may fulfil a challenging role in the evaluation of the majority of primary (benign and malignant) diagnoses as well as metastatic lymph node lesions. Although the morphological features may be quite easily recognized, cytological samples with a scant cellular component may raise some issues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We appraised 263 cytological lymph nodes from different body regions analysed between January and December 2013, including 137 male and 126 female patients, and processed with <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The cytological diagnoses included 160 benign and 103 malignant lesions. We reported 35 benign and 73 malignant lesions from 108 with surgical follow\u2010up. The latter malignant series included 68 metastatic lesions, four suspicious for malignancy and one inadequate sample. The cytological diagnoses were supported by 62 conclusive immunocytochemical and 28 molecular analyses. Of the 108 cases, we documented 35 true negatives, 72 true positives, one false negative and no false positives, resulting in 98.6% sensitivity, 100% specificity, 99% diagnostic accuracy, 97.2% negative predictive value and 100% positive predictive value.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> represents the first diagnostic tool in lymph node management and a reliable approach in order to avoid an excision biopsy. Furthermore, <jats:styled-content style=\"fixed-case\">LBC<\/jats:styled-content> is a feasible method for ancillary tests for which methanol\u2010fixed samples are suitable, such as immunocytochemistry and molecular analysis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12229","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T04:21:34Z","timestamp":1419826894000},"page":"50-58","source":"Crossref","is-referenced-by-count":9,"title":["The potential of liquid\u2010based cytology in lymph node cytological evaluation: the role of morphology and the aid of ancillary techniques"],"prefix":"10.1111","volume":"27","author":[{"given":"E. D.","family":"Rossi","sequence":"first","affiliation":[{"name":"Division of Anatomic Pathology and Histology Universit\u00e0 Cattolica del Sacro Cuore \u2018Agostino Gemelli\u2019 School of Medicine Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Martini","sequence":"additional","affiliation":[{"name":"Division of Anatomic Pathology and Histology Universit\u00e0 Cattolica del Sacro Cuore \u2018Agostino Gemelli\u2019 School of Medicine Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Straccia","sequence":"additional","affiliation":[{"name":"Division of Anatomic Pathology and Histology Universit\u00e0 Cattolica del Sacro Cuore \u2018Agostino Gemelli\u2019 School of Medicine Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Bizzarro","sequence":"additional","affiliation":[{"name":"Division of Anatomic Pathology and Histology Universit\u00e0 Cattolica del Sacro Cuore \u2018Agostino Gemelli\u2019 School of Medicine Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Fadda","sequence":"additional","affiliation":[{"name":"Division of Anatomic Pathology and Histology Universit\u00e0 Cattolica del Sacro Cuore \u2018Agostino Gemelli\u2019 School of Medicine Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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