{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:15:27Z","timestamp":1782940527640,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T00:00:00Z","timestamp":1427241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>We applied immunocytochemistry to fine needle aspiration (<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>) breast lesion slides in an attempt to enhance their objectivity and specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analysed 56 <jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> specimens from patients with histologically confirmed breast lesions, using 34\u03b2E12 and p63 antibodies. Immunostained slides were examined in terms of both solitary positive cells (within clusters and non\u2010clusters) and positive clusters within a slide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Positive scores (\u22652) for p63<jats:sup>+<\/jats:sup> cells and percentages of p63<jats:sup>+<\/jats:sup> clusters differed significantly (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001) between malignant (3 of 34; 9%) and benign (11 of 22; 50%) cases and varied between benign and malignant groups: intraductal papilloma (<jats:styled-content style=\"fixed-case\">IDP<\/jats:styled-content>) (2 of 8), other benign lesions (9 of 14), ductal carcinoma <jats:italic>in\u00a0situ<\/jats:italic> (<jats:styled-content style=\"fixed-case\">DCIS<\/jats:styled-content>) (1 of 11) and invasive carcinoma (<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>) (2 of 23). Similarly, 34\u03b2E12 scores were higher in benign cases (<jats:styled-content style=\"fixed-case\">IDP<\/jats:styled-content>, 8 of 8; other benign, 9 of 14) than in malignant cases (<jats:styled-content style=\"fixed-case\">DCIS<\/jats:styled-content>, 1 of 11; <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>, 3 of 23). As well as a significant difference between benign and malignant cases (17 of 22, 77% versus 4 of 34, 12%; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), the percentage of 34\u03b2E12<jats:sup>+<\/jats:sup> clusters was significantly higher in <jats:styled-content style=\"fixed-case\">IDP<\/jats:styled-content> compared with <jats:styled-content style=\"fixed-case\">DCIS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The immunostaining of <jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> breast specimens for p63 and 34\u03b2E12 may help in difficult diagnoses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12244","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T08:48:38Z","timestamp":1427273318000},"page":"108-114","source":"Crossref","is-referenced-by-count":11,"title":["Immunocytochemical analysis of p63 and 34\u03b2E12 in fine needle aspiration cytology specimens for breast lesions: a potentially useful discriminatory marker between intraductal papilloma and ductal carcinoma <i>in\u00a0situ<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"S.","family":"Hoshikawa","sequence":"first","affiliation":[{"name":"Department of Histopathology and Cytopathology Graduate School of Health Sciences Gunma University Maebashi Gunma Japan"},{"name":"Department of Pathology Gunma University Hospital Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Sano","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Graduate School of Medicine Gunma University Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hirato","sequence":"additional","affiliation":[{"name":"Department of Pathology Gunma University Hospital Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Oyama","sequence":"additional","affiliation":[{"name":"Department of Pathology Gunma University Hospital Maebashi Gunma Japan"},{"name":"Department of Diagnostic Pathology Graduate School of Medicine Gunma University Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Histopathology and Cytopathology Graduate School of Health Sciences Gunma University Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11061"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2012-201109"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199010000-00007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0b013e318235a917"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200301000-00009"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21534"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/RX1W80K68NRJ0PTT"},{"key":"e_1_2_6_9_1","first-page":"18","article-title":"Immunostaining of small cytologic specimens. 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