{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:53:56Z","timestamp":1775523236301,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T00:00:00Z","timestamp":1431907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["NRF\u20102012R1A1A1008668"],"award-info":[{"award-number":["NRF\u20102012R1A1A1008668"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Promoter hypermethylation and the <jats:styled-content style=\"fixed-case\"><jats:italic>BRAF<\/jats:italic><jats:sup>V<\/jats:sup><\/jats:styled-content><jats:sup>600E<\/jats:sup> mutation are both involved in thyroid tumorigenesis. We conducted a pilot study on the diagnosis of thyroid nodules by analysis of promoter hypermethylation status with reference to <jats:styled-content style=\"fixed-case\"><jats:italic>BRAF<\/jats:italic><jats:sup>V<\/jats:sup><\/jats:styled-content><jats:sup>600E<\/jats:sup> mutation and cytopathology results using formalin\u2010fixed, paraffin\u2010embedded (<jats:styled-content style=\"fixed-case\">FFPE<\/jats:styled-content>) tissues and liquid\u2010based preparation (<jats:styled-content style=\"fixed-case\">LBP<\/jats:styled-content>) thyroid fine needle aspiration (<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>) samples to predict more reliably the possibility of papillary carcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We initially performed MethyLight analysis for 30 genes that are known to be hypermethylated in malignancies using 164 papillary carcinomas and 77 benign tissue samples. Five genes selected from the tissue analysis were subsequently analysed in 75 surgically proven benign and 66 surgically proven papillary carcinoma <jats:styled-content style=\"fixed-case\">LBP FNA<\/jats:styled-content> samples. Samples that showed two or more positive results among the five genes were classified as methylation positive. We also analysed the <jats:styled-content style=\"fixed-case\"><jats:italic>BRAF<\/jats:italic><jats:sup>V<\/jats:sup><\/jats:styled-content><jats:sup>600E<\/jats:sup> mutation status of the <jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified five genes that were significantly hypermethylated in malignant tissues: <jats:italic><jats:styled-content style=\"fixed-case\">PTGS<\/jats:styled-content>2<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">HOXA<\/jats:styled-content>1<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">TMEFF<\/jats:styled-content>2<\/jats:italic>,<jats:italic> p16<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content><\/jats:italic>. With respect to diagnostic potential, results obtained using the <jats:styled-content style=\"fixed-case\"><jats:italic>BRAF<\/jats:italic><jats:sup>V<\/jats:sup><\/jats:styled-content><jats:sup>600E<\/jats:sup> mutation test combined with cytological examination were not significantly different from those obtained with cytological examination only. Combining methylation analyses with cytological examination or performing all three tests for diagnoses did not improve significantly the negative predictive values and sensitivity, but a significant decrease in positive predictive value and specificity was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Further studies are needed on larger samples to assess the potential value of methylation analysis of thyroid <jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12248","type":"journal-article","created":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T04:37:03Z","timestamp":1432010223000},"page":"122-130","source":"Crossref","is-referenced-by-count":13,"title":["<scp>DNA<\/scp> methylation analysis for the diagnosis of thyroid nodules \u2013 a pilot study with reference to <scp><i>BRAF<\/i><sup>V<\/sup><\/scp><sup>600E<\/sup> mutation and cytopathology results"],"prefix":"10.1111","volume":"27","author":[{"given":"H.","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital Korea University College of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. K.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital Korea University College of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital Korea University College of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. K.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital Korea University College of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital Korea University College of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21166"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4143\/crt.2013.45.1.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3431"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0339(200010)23:4<233::AID-DC3>3.0.CO;2-L"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.50.11.941"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12085"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.20524"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23869"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000110028"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2012.27.4.407"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-010-9587-z"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700306"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2010.0295"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers3021732"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcma.2012.11.007"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0927"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2009.0274"},{"key":"e_1_2_7_19_1","unstructured":"Ronal A.DeLellis Ricardo V.Lloyd Philipp U.Heitz CharisEng.Pathology and Genetics of Tumours of Endocrine Organs (IARC WHO Classification of Tumours); IARC Press Lyon (France) 2004."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050135"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20195"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.0978"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2006.16.17"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-11-0212"},{"key":"e_1_2_7_25_1","first-page":"3698","article-title":"Frequent epigenetic silencing of the CpG island promoter of RASSF1A in thyroid carcinoma","volume":"62","author":"Schagdarsurengin U","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22110"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1078\/0344-0338-00436"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-9-45"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12248","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T01:03:16Z","timestamp":1696294996000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,18]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1111\/cyt.12248"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12248","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,18]]}}}