{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:01:37Z","timestamp":1760299297948},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T00:00:00Z","timestamp":1455235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"Objective<\/jats:title>The treatment of follicular lymphoma (FL<\/jats:styled-content>) depends on its grade. The current World Health Organization (WHO<\/jats:styled-content>) 2008 Classification of Tumours of Haematopoietic and Lymphoid Tissues recommends the grading of FL<\/jats:styled-content> on histological samples according to the Mann and Berard method, taking into consideration the number of centroblasts. There is no generally accepted method for the grading of FL<\/jats:styled-content> in fine needle aspiration biopsy (FNAB<\/jats:styled-content>) samples. The aim of the present study was to devise a grading system for FL<\/jats:styled-content> in cytological samples.<\/jats:p><\/jats:sec>Methods<\/jats:title>Flow cytometry (FC<\/jats:styled-content>) was performed on 60 FNAB<\/jats:styled-content> samples of patients with primary FL<\/jats:styled-content>. We assumed that FL<\/jats:styled-content> cells larger than reactive T lymphocytes on FC<\/jats:styled-content> histograms corresponded to centroblasts. The percentage of large cells was calculated and compared with histological grade, proliferative activity and number of centroblasts per high\u2010power field (HPF<\/jats:styled-content>) on histological slides, and with survival.<\/jats:p><\/jats:sec>Results<\/jats:title>The histological analysis of lymph nodes revealed 20 patients with high\u2010grade and 40 patients with low\u2010grade FL<\/jats:styled-content>. The percentage of large cells in FNAB<\/jats:styled-content> samples correlated significantly with histological grade (P<\/jats:italic> = 0.02), MIB<\/jats:styled-content>1 status (P<\/jats:italic> < 0.001) and the number of centroblasts per HPF<\/jats:styled-content> (P<\/jats:italic> < 0.001). An age over 60 years and a percentage of large cells over 50% in FNAB<\/jats:styled-content> samples were found to have a statistically significant impact on survival by univariate analysis (P<\/jats:italic> = 0.001 and P<\/jats:italic> = 0.006, respectively).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The percentage of large lymphoma cells in FNAB<\/jats:styled-content> samples of FL<\/jats:styled-content> determined by FC<\/jats:styled-content> can be used as a reliable method for FL<\/jats:styled-content> grading, as it is comparable with the histological grading system.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12319","type":"journal-article","created":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T08:14:52Z","timestamp":1455264892000},"page":"390-397","source":"Crossref","is-referenced-by-count":3,"title":["Grading of follicular lymphoma in cytological samples"],"prefix":"10.1111","volume":"27","author":[{"given":"U.","family":"Klop\u010di\u010d","sequence":"first","affiliation":[{"name":"Department of Cytopathology Institute of Oncology Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Lavren\u010dak","sequence":"additional","affiliation":[{"name":"Department of Cytopathology Institute of Oncology Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Ga\u0161ljevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pathology of the Institute of Oncology Ljubljana Slovenia Ljubljana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bra\u010dko","sequence":"additional","affiliation":[{"name":"Department of Pathology University Clinical Center Ljubljana Zalo\u0161ka cesta 2, Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u017d.","family":"Pohar\u2010Marin\u0161ek","sequence":"additional","affiliation":[{"name":"Department of Cytopathology Institute of Oncology Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Kloboves\u2010Prevodnik","sequence":"additional","affiliation":[{"name":"Department of Cytopathology Institute of Oncology Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.11.3909"},{"key":"e_1_2_5_3_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2900010209"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-07-2298"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq574"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601006"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.5858\/2004-128-863-ACEOFL"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.5858\/2007-131-1084-GOFLCO","article-title":"Grading of follicular lymphoma: comparison of routine histology with immunohistochemistry","volume":"131","author":"Martinez AE","year":"2007","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.2.143"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000331355"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1309\/16UL-W4PX-HRL7-7V88"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326303"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22173"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.5144\/0256-4947.2006.205"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv304"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu264"},{"key":"e_1_2_5_18_1","unstructured":"Slovenian Guidelines for management of lymphoma \u2013 edition 2015. 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