{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T21:42:07Z","timestamp":1725658927704},"reference-count":5,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T00:00:00Z","timestamp":1459123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Papanicolaou (Pap) staining is used with various modifications for the staining of cervical smears. It gives a good contrast with sharp nuclear features which aid in accurate interpretation. However, it utilises ethyl alcohol, which is expensive and difficult to purchase. We have attempted to devise a rapid Pap technique which uses acetic acid instead of ethyl alcohol, and yet provides good staining for correct diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liquid\u2010based cytology samples were collected from 102 patients as a part of routine screening. Two smears were prepared from each sample, which were stained with conventional as well as rapid Pap. In the rapid Pap method, the smears were fixed in methanol and dehydration was performed before and after <jats:styled-content style=\"fixed-case\">OG<\/jats:styled-content>6 with 0.5% acetic acid. The final dehydration was performed with methanol. Smears from both techniques were compared by two independent observers for staining quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>With the rapid Pap stain, nuclear staining was comparable with that of conventional Pap (<jats:italic>P<\/jats:italic> &lt; 0.05). Cytoplasmic staining was optimal in 69.6% of cases; however, the suboptimal staining in the rest of the smears did not pose any diagnostic problem.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The rapid Pap technique is a cost\u2010effective alternative to conventional Pap which also saves time and provides good staining quality without compromising the diagnostic interpretation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12330","type":"journal-article","created":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T07:12:47Z","timestamp":1459149167000},"page":"452-455","source":"Crossref","is-referenced-by-count":5,"title":["Efficacy of modified rapid economic acetic acid\u2010based Papanicolaou stain"],"prefix":"10.1111","volume":"27","author":[{"given":"P.","family":"Bhagat","sequence":"first","affiliation":[{"name":"Department of Cytology and Gynaecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Susheilia","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynaecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynaecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sadhukhan","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynaecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rajwanshi","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynaecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynaecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326034"},{"key":"e_1_2_6_3_1","first-page":"1569","volume-title":"Koss's Diagnostic Cytology and its Histopathologic Bases","author":"Bales CE","year":"2006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000331608"},{"key":"e_1_2_6_5_1","first-page":"99","article-title":"Rapid economic, acetic acid, Papanicolaou stain (REAP)\u2014Is it suitable alternative to standard PAP stain?","volume":"1","author":"Biswas RR","year":"2008","journal-title":"Al Ameen J Med Sci"},{"key":"e_1_2_6_6_1","first-page":"484","article-title":"Comparison of the routine Papanicolaou staining technique with the rapid, economic, acetic acid, Papanicolaou (REAP) technique","volume":"3","author":"Asthana A","year":"2014","journal-title":"Int J Med and Dent Sci"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12330","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12330","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T23:43:39Z","timestamp":1696290219000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,28]]},"references-count":5,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/cyt.12330"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12330","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,28]]}}}