{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T22:15:42Z","timestamp":1722982542890},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,6]],"date-time":"2016-04-06T00:00:00Z","timestamp":1459900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The Breast Marker Cocktail from Biocare Medical comprises five antibodies recognising p63, and cytokeratins (<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>s) 7, 18, 5 and 14. Immunohistochemistry using this cocktail is useful for diagnosing proliferative intraductal breast lesions. However, cytology using the cocktail has not been reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We report 139 cases of mammary samples collected by fine needle aspiration (<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>) for which histological diagnoses were available. After cell transfer, immunocytochemistry was performed using the cocktail, and clusters of cells were classified. A cluster with no or limited <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>5\/14 expression (&lt;20% of cells) was classified as a monotonous cluster. One with more than 20% of cells showing <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>5\/14 expression was defined as a mosaic cluster. When at least one p63\u2010positive cell was present, we defined it as a cluster with p63. We also evaluated background p63\u2010positive myoepithelial cell densities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The diagnostic sensitivity and specificity for carcinomas were 97.8% (89\/91) and 91.7% (11\/12), respectively, using the criterion of two or more monotonous clusters lacking p63. Two false\u2010negative cases were triple\u2010negative cancers; one false\u2010positive was an apocrine papilloma. The numbers of monotonous clusters with p63 differed significantly between benign lesions, ductal carcinoma <jats:italic>in situ<\/jats:italic> (<jats:styled-content style=\"fixed-case\">DCIS<\/jats:styled-content>)\/lobular carcinoma <jats:italic>in situ<\/jats:italic> (<jats:styled-content style=\"fixed-case\">LCIS<\/jats:styled-content>) and invasive carcinomas (<jats:italic>P<\/jats:italic> &lt; 0.001). The background myoepithelial cell density was significantly higher in fibroepithelial tumours than in other lesions (<jats:italic>P<\/jats:italic> &lt; 0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Immunocytochemistry using this antibody cocktail showed good sensitivity and specificity for diagnosing breast cancers. Thus, this method is useful for mammary cytology using <jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12335","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T13:38:24Z","timestamp":1460295504000},"page":"465-471","source":"Crossref","is-referenced-by-count":6,"title":["Usefulness of immunocytochemistry using a Breast Marker antibody cocktail targeting P63\/cytokeratin7\/18\/cytokeratin5\/14 for fine needle aspiration of the breast: a retrospective cohort study of 139 cases"],"prefix":"10.1111","volume":"27","author":[{"given":"S.","family":"Tanaka","sequence":"first","affiliation":[{"name":"Division of Infectious Disease Pathology Kobe University Graduate School of Medicine Kobe Hyogo Japan"},{"name":"Department of Pathology Yodogawa Christian Hospital Osaka Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Kanomata","sequence":"additional","affiliation":[{"name":"Department of Pathology Kawasaki Medical School Kurashiki Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Teramura","sequence":"additional","affiliation":[{"name":"Department of Pathology Yodogawa Christian Hospital Osaka Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Wakita","sequence":"additional","affiliation":[{"name":"Chayamachi Breast Clinic Osaka Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Kunihisa","sequence":"additional","affiliation":[{"name":"Department of Surgery Yodogawa Christian Hospital Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Yano","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease Pathology Kobe University Graduate School of Medicine Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Moriya","sequence":"additional","affiliation":[{"name":"Department of Pathology Kawasaki Medical School Kurashiki Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease Pathology Kobe University Graduate School of Medicine Kobe Hyogo Japan"},{"name":"Division of Molecular Medicine and Medical Genetics Department of Pathology Kobe University Graduate School of Medicine Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(97)00192-X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19980515)82:10<1867::AID-CNCR8>3.0.CO;2-K"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.66"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2014.08.007"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000017371.72714.C5"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2000.00882.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200108000-00010"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181aec446"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12282-009-0127-7"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.12.936"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11061"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1309\/RX1W80K68NRJ0PTT"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2009.00713.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.5795\/jjscc.54.170"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21063"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22897"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200301000-00009"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-006-0309-8"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e31803244d5"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2006"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90400-7"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/34.7.2423579"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199010000-00007"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000157941.88161.39"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2006.02069.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1559"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800570"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2007.02902.x"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31820598a2"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPAH5WR2LFTUSI"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12335","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12335","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T23:44:32Z","timestamp":1696290272000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,6]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/cyt.12335"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12335","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,6]]}}}