{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T01:27:50Z","timestamp":1760318870893},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T00:00:00Z","timestamp":1471305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004694","name":"Kaohsiung Medical University","doi-asserted-by":"publisher","award":["KMUH104\u20104M59"],"award-info":[{"award-number":["KMUH104\u20104M59"]}],"id":[{"id":"10.13039\/501100004694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the utility of a proposed cell transfer technique for constructing cytological smear microarrays and its potential applications in multiplex immunocytochemical (<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>) staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ninety\u2010six cytology smears, including two pericardial effusions, 22 ascites and 72 pleural effusions, were transferred to a 33\u2010plex cytological microarray. Paired staining of thyroid transcription factor\u20101 (<jats:styled-content style=\"fixed-case\">TTF<\/jats:styled-content>\u20101) and calretinin <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> was performed in duplicate slides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Most of the smeared cells selected for transfer could be removed from the original slides with a minimal loss of cells and with no change in morphological features or immunoreactivity. Comparison of the staining results with immunohistochemical staining results, clinical history and histopathological reports available for each patient revealed that <jats:styled-content style=\"fixed-case\">TTF<\/jats:styled-content>\u20101 was positive in 32\/33 metastatic pulmonary adenocarcinomas (<jats:styled-content style=\"fixed-case\">PAC<\/jats:styled-content>s), 1\/15 non\u2010pulmonary adenocarcinomas and 0\/45 benign effusions. The <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> results for <jats:styled-content style=\"fixed-case\">TTF<\/jats:styled-content>\u20101 on a transferred cytological microarray revealed high (97%) sensitivity and high (96.7%) specificity for the detection of metastatic <jats:styled-content style=\"fixed-case\">PAC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Cytology microarrays can be constructed by transferring cells from serous fluid cytological smears, and cells transferred to the microarray retain their morphological integrity and immunoreactivity. Researchers can use the technique for simultaneous immunostaining of multiple specimens in studies of neoplastic or non\u2010neoplastic diseases when available tissue samples are limited.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12360","type":"journal-article","created":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T05:42:44Z","timestamp":1471326164000},"page":"157-163","source":"Crossref","is-referenced-by-count":4,"title":["Cell transfer technique for constructing cytological microarrays for immunocytochemical analysis"],"prefix":"10.1111","volume":"28","author":[{"given":"C.\u2010H.","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Pathology Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u2010H.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.\u2010L.","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Pathology Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010F.","family":"Kuo","sequence":"additional","affiliation":[{"name":"Medical Devices Development Section Metal Industries Research &amp; Development Centre Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010J.","family":"Chen","sequence":"additional","affiliation":[{"name":"Medical Devices Development Section Metal Industries Research &amp; Development Centre Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u2010Y.","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Pathology Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung Taiwan"},{"name":"Department of Pathology Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung Taiwan"},{"name":"Graduate Institute of Medicine College of Medicine Kaohsiung Medical University Kaohsiung Kaohsiung Taiwan"},{"name":"Institute of Biomedical Sciences National Sun Yat\u2010Sen University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,16]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"259","article-title":"Restoration of broken cytology slides and creation of multiple slides from a single smear preparation","volume":"36","author":"Brown GG","year":"1992","journal-title":"Acta Cytol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21735"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22897"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21063"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199805)18:5<377::AID-DC16>3.0.CO;2-F"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-6646(09)60196-0"},{"key":"e_1_2_8_8_1","first-page":"18","article-title":"Immunostaining of small cytologic specimens. 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