{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T15:04:58Z","timestamp":1780499098872,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T00:00:00Z","timestamp":1471305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Biliary brush cytology is an important diagnostic tool in the evaluation of pancreatobiliary malignancies. However, it is difficult to distinguish between malignant and benign cells. The present study evaluated the utility of immunocytochemical expression of Claudin\u201018 and Maspin in brushing cytology specimens of pancreatobiliary lesions in the diagnosis of pancreatobiliary malignancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study retrospectively assessed biliary and pancreatic duct brushing cytology specimens of 43 patients whose pancreatobiliary lesions were histologically diagnosed at the University of Miyazaki Hospital. Scanty cellularity slides and cases with no histological confirmation were excluded. Alcohol\u2010fixed and Papanicolaou\u2010stained slides were immunostained with monoclonal antibodies to Claudin\u201018 and Maspin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 43 patients, 35 (81.4%) were finally histologically diagnosed with invasive adenocarcinomas. The sensitivity of routine cytology for the detection of malignancy was 63%, and the specificity was 100%. The sensitivity of cytology in combination with immunocytochemical expression of Claudin\u201018 (89%) or Claudin\u201018 and\/or Maspin (97%) was significantly higher than that of cytology alone (<jats:italic>P<\/jats:italic> &lt; 0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Immunocytochemical staining for Claudin\u201018 and Maspin improved the diagnostic sensitivity for pancreatobiliary adenocarcinomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12368","type":"journal-article","created":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T05:46:46Z","timestamp":1471326406000},"page":"116-121","source":"Crossref","is-referenced-by-count":20,"title":["Immunocytochemistry for Claudin\u201018 and Maspin in biliary brushing cytology increases the accuracy of diagnosing pancreatobiliary malignancies"],"prefix":"10.1111","volume":"28","author":[{"given":"T.","family":"Tokumitsu","sequence":"first","affiliation":[{"name":"Division of Pathology Faculty of Medicine University of Miyazaki Hospital Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Pathology Faculty of Medicine University of Miyazaki Hospital Miyazaki Japan"},{"name":"Department of Diagnostic Pathology Faculty of Medicine University of Miyazaki Hospital Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medicine Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Moriguchi\u2010Goto","sequence":"additional","affiliation":[{"name":"Division of Pathology Faculty of Medicine University of Miyazaki Hospital Miyazaki Japan"},{"name":"Department of Diagnostic Pathology Faculty of Medicine University of Miyazaki Hospital Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Kondo","sequence":"additional","affiliation":[{"name":"Community Medical Center Faculty of Medicine Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Nanashima","sequence":"additional","affiliation":[{"name":"Division of Hepato\u2010Biliary\u2010Pancreas Surgery Department of Surgery Faculty of Medicine University of Miyazaki Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Asada","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medicine Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,16]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"498","article-title":"Brush cytology of the extrahepatic biliary tract: comparison of cytologic features of adenocarcinoma and benign biliary strictures","volume":"8","author":"Cohen MB","year":"1995","journal-title":"Mod Pathol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2004.00133.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2007.00447.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.07020.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.511"},{"key":"e_1_2_6_7_1","first-page":"6265","article-title":"Claudin\u20104 expression decreases invasiveness and metastatic potential of pancreatic cancer","volume":"63","author":"Michl P","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_8_1","first-page":"2567","article-title":"Tight junction proteins claudin\u20103 and claudin\u20104 are frequently overexpressed in ovarian cancer but not in ovarian cystadenomas","volume":"9","author":"Rangel LB","year":"2003","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3727\/096504001108747477"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.21.7380-7390.2001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12182"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-007-0448-x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-015-9990-z"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8290962"},{"key":"e_1_2_6_15_1","first-page":"393","article-title":"Expression of maspin in endometrioid adenocarcinoma of endometrium","volume":"17","author":"Li HW","year":"2007","journal-title":"Oncol Rep"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-014-1705-4"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2004.00129.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21842"},{"key":"e_1_2_6_19_1","first-page":"18","article-title":"Immunostaining of small cytologic specimens. 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