{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:51:48Z","timestamp":1771462308940,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T00:00:00Z","timestamp":1494806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004711","name":"Chung Hua University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Salivary gland cytology is challenging because it includes a diversity of lesions and a wide spectra of tumours. Recently, it has been reported that many types of salivary gland tumours have specific molecular diagnostic signatures that could be identified by <jats:styled-content style=\"fixed-case\">fluorescent in\u2010situ hybridisation (FISH)<\/jats:styled-content>. The aim of the present study was to demonstrate the feasibility and efficiency of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> on routine cytological salivary gland smears.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> was conducted on 37 cytological salivary gland smears from 34 patients. According to the cytological diagnosis suspected, <jats:italic><jats:styled-content style=\"fixed-case\">MECT<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">MAML<\/jats:styled-content>2<\/jats:italic> gene fusion and rearrangements of <jats:italic><jats:styled-content style=\"fixed-case\">PLAG<\/jats:styled-content>1<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content><\/jats:italic>, or <jats:italic><jats:styled-content style=\"fixed-case\">ETV<\/jats:styled-content>6<\/jats:italic> were analysed. The presence and percentages of cells that had gene rearrangements were evaluated. Results were compared with the histological surgical samples, available from 26 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">The <jats:italic>PLAG<\/jats:italic><\/jats:styled-content><jats:italic>1<\/jats:italic> rearrangement was observed in 12\/20 (60%) cases of pleomorphic adenoma. <jats:italic><jats:styled-content style=\"fixed-case\">MECT<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">MAML<\/jats:styled-content>2<\/jats:italic> gene fusion was observed in 1:2 mucoepidermoid carcinomas but was not observed in five other tumours (two pleomorphic adenomas, one Warthin's tumour, one mammary analogue secretory carcinoma [<jats:styled-content style=\"fixed-case\">MASC<\/jats:styled-content>] and one cystic tumour). <jats:italic><jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content><\/jats:italic> rearrangement was observed in 4\/4 adenoid cystic carcinomas. <jats:italic><jats:styled-content style=\"fixed-case\">ETV<\/jats:styled-content>6<\/jats:italic>\u2010gene splitting identified one <jats:styled-content style=\"fixed-case\">MASC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> had a specificity of 100% and a sensitivity of 66.7%. When <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> and cytological analyses were combined, the overall sensitivity was increased to 93.3%. It can thus be concluded that when the <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> analysis is positive, the extent of surgery could be determined with confidence pre\u2010operatively without needing a diagnosis from a frozen section.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12427","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T09:03:12Z","timestamp":1494838992000},"page":"312-320","source":"Crossref","is-referenced-by-count":36,"title":["Use of fluorescent in\u2010situ hybridisation in salivary gland cytology: A powerful diagnostic tool"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9669-8316","authenticated-orcid":false,"given":"S. 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