{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:57:14Z","timestamp":1782277034146,"version":"3.54.5"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T00:00:00Z","timestamp":1514332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81402320"],"award-info":[{"award-number":["81402320"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Precise cytological diagnosis of pelvic high\u2010grade serous carcinoma (<jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content>) in ascites is important for tumour staging, therapeutic decision\u2010making and prognostic evaluation. However, it can often be difficult to distinguish metastatic <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content> cells from reactive mesothelial cells based on morphology alone. Immunocytochemical analysis of ascites cell blocks has been used to obtain accurate diagnosis and provide a reliable basis for treatment decisions in the clinic. This study was performed to determine whether a panel of antibodies is necessary to achieve high specificity and sensitivity for the identification of <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ascites samples from 70 cases (70\/253, 27.7%) of histologically confirmed <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content> were postoperatively collected from 2012 to 2015 and were immunocytochemically analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sensitivity and specificity of Ber\u2010<jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content>4 (a marker of <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content>) for detecting <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content> was 85.7% and 82.1%, respectively, whereas the sensitivity and specificity of <jats:styled-content style=\"fixed-case\">HBME<\/jats:styled-content>\u20101 for identifying mesothelial cells was 100% and 68.3%, respectively. To improve the rate of detection further of <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content>, 29 cases of ascites were also stained for E\u2010cadherin (a marker of <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content>) and calretinin (a marker of mesothelial cells). The combination of Ber\u2010<jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content>4 and E\u2010cadherin as markers of adenocarcinoma cells increased the sensitivity and specificity for <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content> detection to 100% and 88.9%, respectively. Meanwhile, the sensitivity and specificity for mesothelial cell identification increased to 100% and 90%, respectively, when <jats:styled-content style=\"fixed-case\">HBME<\/jats:styled-content>\u20101 and calretinin were combined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This panel of complementary biomarkers is valuable and ideal for the differential diagnosis of <jats:styled-content style=\"fixed-case\">HGSC<\/jats:styled-content> based on ascites cytology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12514","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T09:59:13Z","timestamp":1514368753000},"page":"247-253","source":"Crossref","is-referenced-by-count":7,"title":["Improving the cytological diagnosis of high\u2010grade serous carcinoma in ascites with a panel of complementary biomarkers in cell blocks"],"prefix":"10.1111","volume":"29","author":[{"given":"X.","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cytology Tianjin Medical University Clinical Hospital of Gynecology Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3128-3472","authenticated-orcid":false,"given":"L.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Central Hospital of Gynecology and Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cytology Tianjin Medical University Clinical Hospital of Gynecology Obstetrics Tianjin China"},{"name":"Department of Pathology Tianjin Central Hospital of Gynecology and Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cytology Tianjin Medical University Clinical Hospital of Gynecology Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cytology Tianjin Medical University Clinical Hospital of Gynecology Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Central Hospital of Gynecology and Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology QEII Capital Health Dalhousie University Halifax NS Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomic Pathology William Beaumont Hospital Royal Oak MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cytology Tianjin Medical University Clinical Hospital of Gynecology Obstetrics Tianjin China"},{"name":"Department of Pathology Tianjin Central Hospital of Gynecology and Obstetrics Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,27]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.376"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2644"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/367103"},{"key":"e_1_2_5_5_1","first-page":"236","article-title":"Ascites in ovarian carcinoma \u2010 reliability and limitations of cytological analysis","volume":"64","author":"Zivadinovic R","year":"2015","journal-title":"West Indian Med J"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.11.018"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.5858\/133.5.739","article-title":"Challenges in the interpretation of peritoneal cytologic specimens","volume":"133","author":"Lin O","year":"2009","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21489"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.9044"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPHSUJ15NEYMIP"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2000.00770.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(200005)22:5<263::AID-DC1>3.0.CO;2-4"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2007.00482.x"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcyt.12514","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12514","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T13:06:41Z","timestamp":1695474401000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12514"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,27]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1111\/cyt.12514"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12514","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,27]]}}}