{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:35:34Z","timestamp":1787841334201,"version":"build-2784847793"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T00:00:00Z","timestamp":1525651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K08355"],"award-info":[{"award-number":["15K08355"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Positive peritoneal cytology (<jats:styled-content style=\"fixed-case\">PPC<\/jats:styled-content>) in endometrial cancer remains a controversial topic. Cleaved caspase\u20103 (<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3) and Ki\u201067 are excellent markers of apoptotic and proliferating cells, respectively. The objective of this study was to determine the significance of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3 and Ki\u201067 expression in peritoneal cytology samples as prognostic factors for endometrial cancer with <jats:styled-content style=\"fixed-case\">PPC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty endometrial cancer specimens with <jats:styled-content style=\"fixed-case\">PPC<\/jats:styled-content> alone were divided into 51 endometrioid tumours (43 endometrioid carcinomas and eight carcinomas with squamous differentiation) and nine non\u2010endometrioid tumours (two serous carcinomas, three clear cell carcinomas and four carcinosarcomas). <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3 and Ki\u201067 expression in peritoneal cytology samples were immunocytochemically assessed and correlated with disease\u2010free survival (<jats:styled-content style=\"fixed-case\">DFS<\/jats:styled-content>) and overall survival (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression levels of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3 and Ki\u201067 were not associated with any clinicopathological parameter. Patients with non\u2010endometrioid tumours had significantly shorter <jats:styled-content style=\"fixed-case\">DFS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.001) and <jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.001). Low <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3 expression (<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3<jats:sup>Low<\/jats:sup>) was significantly associated with shorter <jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.02), but not <jats:styled-content style=\"fixed-case\">DFS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.13). Multivariate analysis showed that non\u2010endometrioid histology and <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3<jats:sup>Low<\/jats:sup> were independent prognostic factors. However, Ki\u201067 expression was not associated with survival. When endometrioid and non\u2010endometrioid tumours were assessed separately, <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3<jats:sup>Low<\/jats:sup> was significantly associated with shorter <jats:styled-content style=\"fixed-case\">DFS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.002) and <jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>.002) in patients with non\u2010endometrioid tumours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3<jats:sup>Low<\/jats:sup> in peritoneal cytology samples is a poor prognostic factor in patients with endometrial cancers, especially non\u2010endometrioid tumours. Immunocytochemical analysis of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>3 expression could potentially facilitate identification of patients with high\u2010risk endometrial cancer with <jats:styled-content style=\"fixed-case\">PPC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12550","type":"journal-article","created":{"date-parts":[[2018,4,7]],"date-time":"2018-04-07T06:40:16Z","timestamp":1523083216000},"page":"254-261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cleaved caspase\u20103 expression is a potential prognostic factor for endometrial cancer with positive peritoneal cytology"],"prefix":"10.1111","volume":"29","author":[{"given":"N.","family":"Ogane","sequence":"first","affiliation":[{"name":"Department of Pathology Kanagawa Prefectural Ashigarakami Hospital Matsuda 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