{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:27:51Z","timestamp":1761989271548,"version":"3.37.3"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T00:00:00Z","timestamp":1539302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate the diagnostic yield of the cell block (<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>) technique with immunohistochemistry in patients with mesenchymal neoplasms of the gastrointestinal tract collected by endoscopic ultrasound\u2010guided fine\u2010needle aspiration (<jats:styled-content style=\"fixed-case\">EUS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tissue samples from consecutive patients with subepithelial lesions collected by <jats:styled-content style=\"fixed-case\">EUS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content>, without analysis by on\u2010site cytopathology, were evaluated by the same pathologist only using <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">AAF<\/jats:styled-content> fixative. Sections were stained with haematoxylin\u2010eosin and underwent complementary immunohistochemical staining for <jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CD<\/jats:styled-content>117, <jats:styled-content style=\"fixed-case\">DOG<\/jats:styled-content>\u20101 and S100 in the presence of mesenchymal neoplasms. Specimens were defined as diagnostic when sufficient tissue was present for histopathological evaluation and immunohistochemistry analysis. If they were insufficient for complete evaluation, the specimens were considered nondiagnostic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Between September 2012 and December 2016, a total of 158 patients (median age: 57\u00a0years, 64.5% women) underwent <jats:styled-content style=\"fixed-case\">EUS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> with an average of three needle passes for every lesion. The median lesion size was 17\u00a0mm. There were 113 mesenchymal neoplasms confirmed by immunohistochemistry (66 leiomyomas, 44 <jats:styled-content style=\"fixed-case\">GIST<\/jats:styled-content>s, two schwannomas, one leiomyosarcoma). The overall diagnostic yield of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s was 84.17%. However, diagnosis was obtained in 98.5% (133\/135) of the cases after exclusion of 23 cases in which <jats:styled-content style=\"fixed-case\">EUS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> sampling was insufficient or without tumoural tissue. Only two mesenchymal neoplasms were not confirmed by <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s even after immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s collected by <jats:styled-content style=\"fixed-case\">EUS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FNA<\/jats:styled-content> and analysed by immunohistochemistry showed a high diagnostic yield in patients with mesenchymal neoplasms, even without on\u2010site cytopathology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12630","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T13:13:26Z","timestamp":1537103606000},"page":"531-536","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Differential diagnosis of mesenchymal neoplasms of the digestive tract by cell 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Atal\u00edbio","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Pathology Santa Casa Hospital Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i4.439"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2005.05.001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/0102-672020180001e1350"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/2303-9027.208173"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00464-015-4494-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4253\/wjge.v7.i11.1014"},{"key":"e_1_2_6_8_1","first-page":"246","article-title":"Cell block technique and cytological smears for the differential diagnosis of pancreatic neoplasms after endosonography\u2010guided 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