{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:36:42Z","timestamp":1787845002766,"version":"build-2784847793"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p>\n                    Serous effusion fluid is one of the most commonly encountered specimens in routine cytopathology practice. It provides invaluable information about the patient and the clinical status; but to get the most of it, specimen handling and processing must be carried out properly. Cytomorphology is the basis of a successful analysis which should complemented by ancillary tests when needed. A wide spectrum of ancillary techniques \u2010 ranging from immunocytochemistry and flow cytometry to different assays of molecular pathology \u2010 can be applied to serous effusions. This article describes the acquisition and management of serous effusion fluids, methods for preservation and transportation, different techniques of cytopreparation, application of immunocytochemistry, flow cytometry, and fluorescence in\u2010situ hybridization (\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    ), as well as\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    extraction for polymerase chain reaction (\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    ) and next generation sequencing (\n                    <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>\n                    ). Principles of bio\u2010banking of effusion samples are also discussed which is getting more important in correlation with the developments in personalized medicine.\n                  <\/jats:p>","DOI":"10.1111\/cyt.12654","type":"journal-article","created":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T14:10:22Z","timestamp":1542291022000},"page":"31-38","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Management of cytological material, pre\u2010analytical procedures and bio\u2010banking in effusion cytopathology"],"prefix":"10.1111","volume":"30","author":[{"given":"Marianne","family":"Engels","sequence":"first","affiliation":[{"name":"Institute of Pathology University Hospital of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Michael","sequence":"additional","affiliation":[{"name":"Department of Pathology Case Western Reserve University\/University Hospitals Cleveland Health Center Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Dobra","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Hjerpe","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambrogio","family":"Fassina","sequence":"additional","affiliation":[{"name":"Department of Medicine Surgical Pathology &amp; Cytopathology Unit University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4278-722X","authenticated-orcid":false,"given":"Pinar","family":"Firat","sequence":"additional","affiliation":[{"name":"Department of Pathology Koc University School of Medicine Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/pp-2016-0001"},{"key":"e_1_2_6_3_1","first-page":"93","volume-title":"Manual of Pleural Procedures","author":"Colt HC","year":"1999"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21452"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21790"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21850"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20587"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369198"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23567"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21518"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0125-RA"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12216"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21814"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP83ADULCXMAIX"},{"issue":"4","key":"e_1_2_6_15_1","first-page":"198","article-title":"[Diagnostic and predictive analyses of cytological specimens of non\u2010small cell lung cancer: Strategies and challenges]. 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