{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T11:36:08Z","timestamp":1759836968075,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T00:00:00Z","timestamp":1560902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>DNA<\/jats:styled-content> methylation has been suggested as one of the epigenetic changes promoting carcinogenesis. The aim of this study was to prospectively evaluate the methylation status of CADM<\/jats:styled-content> 1, MAL<\/jats:styled-content> and hsa\u2010miR\u2010124 genes in high\u2010grade squamous intraepithelial lesion (HSIL<\/jats:styled-content>) liquid\u2010based cytology (LBC<\/jats:styled-content>) samples with a histological correlation.<\/jats:p><\/jats:sec>Methods<\/jats:title>Seventy histologically confirmed cases of HSIL<\/jats:styled-content> paired with prior screening LBC<\/jats:styled-content> diagnosis of HSIL<\/jats:styled-content> within a 3\u2010month interval were selected. Histologically, the lesions were reviewed and assessed including: (a) number of blocks harbouring dysplastic squamous epithelium; (b) number of blocks containing glandular extension of dysplastic epithelium; and (c) the depth of glandular extension (which was assessed semi\u2010quantitatively as graded 1\u20103). Human papillomavirus (HPV)<\/jats:styled-content> subtyping was performed from residual LBC<\/jats:styled-content> materials using the LINEAR ARRAY HPV<\/jats:styled-content> Genotyping Test and in\u2010house polymerase chain reaction targeting the HPV<\/jats:styled-content> E1 gene. The detection of methylation silencing of tumour suppressor genes CADM<\/jats:styled-content>1, MAL<\/jats:styled-content> and hsa\u2010miR\u2010124 was performed by multiplex methylation\u2010specific real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec>Results<\/jats:title>A positive methylation status was detected in 41 cases (58.6%). The number of blocks with HSIL<\/jats:styled-content> varied from one to 13. Glandular extension was seen in 44 cases with the number of blocks involved ranging from one to 10. The depth of HSIL<\/jats:styled-content> glandular extension varied.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The DNA<\/jats:styled-content> methylation test allows HSIL<\/jats:styled-content> lesions to be divided into two distinct groups of methylated <\/jats:italic>HSIL<\/jats:styled-content> in significantly older patients and unmethylated <\/jats:italic>HSIL<\/jats:styled-content> in younger patients. This study was not able to prove that methylation status in cervical HSIL<\/jats:styled-content> correlates with the size of the lesion (measured by the number of blocks involved) or with HSIL<\/jats:styled-content> propensity for endocervical glandular extension, nor with HPV<\/jats:styled-content> type or multi\u2010infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12718","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T06:48:36Z","timestamp":1557470916000},"page":"426-431","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The detection of DNA<\/scp> methylation of tumour suppressor genes in cervical high\u2010grade squamous intraepithelial lesion: A prospective cytological\u2010histological correlation study of 70 cases"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4038-5641","authenticated-orcid":false,"given":"Ondrej","family":"Ondi\u010d","sequence":"first","affiliation":[{"name":"Department of Pathology Medical Faculty Charles University Hospital Pilsen Charles University Pilsen Czech Republic"},{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"N\u011bmcov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical Faculty Charles University Hospital Pilsen Charles University Pilsen Czech Republic"},{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Alaghehbandan","sequence":"additional","affiliation":[{"name":"Department of Pathology Royal Columbian Hospital University of British Columbia Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate\u0159ina","family":"\u010cern\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical Faculty Charles University Hospital Pilsen Charles University Pilsen Czech Republic"},{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Gomol\u010d\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Kinkorov\u00e1\u2010Lu\u0148\u00e1\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Chytra","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrieta","family":"\u0160idlov\u00e1","sequence":"additional","affiliation":[{"name":"Cytopathos, s.r.o. 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