{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:14:13Z","timestamp":1790352853632,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T00:00:00Z","timestamp":1563494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002468","name":"Dong-A University","doi-asserted-by":"publisher","award":["2016"],"award-info":[{"award-number":["2016"]}],"id":[{"id":"10.13039\/501100002468","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Programmed cell death ligand\u20101 (<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1) has emerged as a predictive biomarker in lung cancer. <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) assay predicts the response to immunotherapy, but cytology specimens are often the only samples available in a considerable proportion of advanced lung cancer patients. We delineate practical feasibility and efficacy of cytology cell\u2010block (<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>) specimens for <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression and concordance between cytology <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s and surgical resection specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, 58 eligible patients with primary lung cancer who received computed tomography\u2010guided percutaneous needle aspiration and surgery were included. <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> (clone <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>263) was performed on <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s prepared from residual liquid\u2010based cytology material and matched surgical resection specimens. <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 positive tumour cell proportion was categorised in four score groups: (a) &lt;1%; (b) \u22641% to &lt;10%; (c) \u226410% to &lt;50%, (d) \u226550%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparison of <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression in cytology <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s and matched surgical resection specimens showed a high concordance (\u03ba value 0.65). According to the therapeutic guideline of immunotherapeutic agents, a positive percent agreement was 94.34%, and a negative percent agreement was 100% at a cut\u2010off value for positivity of 1% <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression. There was a significant difference observed with regard to rates of <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 positivity when comparing smoking history (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.02), age (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.04) and pathological <jats:styled-content style=\"fixed-case\">TNM<\/jats:styled-content> stage (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.04).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results show that cytology <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s evaluated for <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> assay have high concordance with matched surgical resection specimens and can be used for assessing <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression. Also, we propose that <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>s are suitable materials for evaluating <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression while simultaneously performing both diagnostic and molecular tests.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12743","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T07:09:28Z","timestamp":1560841768000},"page":"578-585","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Cell\u2010blocks are suitable material for programmed cell death ligand\u20101 immunohistochemistry: Comparison of cell\u2010blocks and matched surgical resection specimens in lung cancer"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6142-4569","authenticated-orcid":false,"given":"Min Gyoung","family":"Pak","sequence":"first","affiliation":[{"name":"Department 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