{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:54:14Z","timestamp":1777596854360,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["JP 26460459"],"award-info":[{"award-number":["JP 26460459"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>To investigate the relationship between the morphological features of nuclear enlarged cells and human papillomavirus (HPV<\/jats:styled-content>) infection in negative for intraepithelial lesion or malignancy (NILM<\/jats:styled-content>) and atypical squamous cells of undetermined significance (ASC<\/jats:styled-content>\u2010US<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>In total, 128 Papanicolaou specimens comprising 41 ASC<\/jats:styled-content>\u2010US<\/jats:styled-content> cases and 87 NILM<\/jats:styled-content> cases were examined. Cell morphological analysis was performed using both area ratio (nuclear area in cells with nuclear enlargement\/nuclear area in normal intermediate cells) and nuclear contour thickening. High\u2010risk HPV<\/jats:styled-content> was detected using the Uniplex E6\/E7 polymerase chain reaction assay and logistic regression analyses of factors related to high\u2010risk HPV<\/jats:styled-content> infection were performed.<\/jats:p><\/jats:sec>Results<\/jats:title>Nuclear contour thickening was present in 57.7% (64\/111 cells) of high\u2010risk HPV<\/jats:styled-content> positive cases and 21.8% (69\/317 cells) of high\u2010risk HPV<\/jats:styled-content> negative cases. There was a statistically significant association (P\u00a0=\u00a0<\/jats:italic>0.01) between high\u2010risk HPV<\/jats:styled-content> infection and nuclear contour thickening. Nuclear contour thickening was approximately one\u2010third higher in NILM<\/jats:styled-content> cases than in ASC<\/jats:styled-content>\u2010US<\/jats:styled-content> cases (odds ratio, 0.371; 95% confidence interval, 0.208\u20130.662) and three times higher in high\u2010risk HPV\u2010<\/jats:styled-content>positive cases than in high\u2010risk HPV<\/jats:styled-content>\u2010negative cases (odds ratio<\/jats:styled-content>, 2.831; 95% confidence interval, 1.591\u20135.039).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Our results suggest that nuclear contour thickening in nuclear enlarged cells in NILM<\/jats:styled-content> and ASC<\/jats:styled-content>\u2010US<\/jats:styled-content> cases may be a cellular finding associated with HPV<\/jats:styled-content> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12756","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T16:08:55Z","timestamp":1563293335000},"page":"644-649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A study of the relationship between nuclear contour thickening, nuclear enlargement and human papillomavirus infection in squamous cells"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6367-2910","authenticated-orcid":false,"given":"Junko","family":"Iijima","sequence":"first","affiliation":[{"name":"Department of Medical technology Faculty of Health Sciences Kyorin University Mitaka Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Okayama","sequence":"additional","affiliation":[{"name":"School of Medical Technology Faculty of Health Science Gunma Paz University Takasaki Gunma Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Teruya","sequence":"additional","affiliation":[{"name":"Department of Health and Welfare Faculty of Health Sciences Kyorin University Mitaka Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Hata","sequence":"additional","affiliation":[{"name":"Department of Medical technology Faculty of Health Sciences Kyorin University Mitaka Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natsuko","family":"Shiina","sequence":"additional","affiliation":[{"name":"I<\/i> LABO CytoSTD Laboratory, Inc. Hachioji Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Yabusaki","sequence":"additional","affiliation":[{"name":"I<\/i> LABO CytoSTD Laboratory, Inc. Hachioji Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuaki","family":"Okodo","sequence":"additional","affiliation":[{"name":"Department of Medical technology Faculty of Health Sciences Kyorin University Mitaka Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,22]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"31","volume-title":"The Bethesda System for Reporting Cervical Cytology: definitions, Criteria, and Explanatory Notes","author":"Kurtycz DFI","year":"2015"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000323320"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325116"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/030089160609200305"},{"key":"e_1_2_10_6_1","first-page":"104","volume-title":"The Bethesda System for Reporting Cervical Cytology: definitions, Criteria, and Explanatory Notes","author":"Abdul\u2010Karim FW","year":"2015"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326933"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2840110402"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22842"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25017"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24323"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335562"},{"key":"e_1_2_10_14_1","first-page":"68","author":"Frost JK","year":"1991","journal-title":"Comprehensive Cytopathplogy"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.5795\/jjscc.24.51"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.5795\/jjscc.26.551"},{"key":"e_1_2_10_17_1","first-page":"383","article-title":"Origin of the koilocyte in condylomata of the human cervix. 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