{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:05:39Z","timestamp":1785679539233,"version":"3.56.0"},"reference-count":11,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:00:00Z","timestamp":1567987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the utility of claudin\u20104 as a pan\u2010carcinoma marker in cell\u2010blocks of effusion specimens and compare results with Ber\u2010Ep4 staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Effusion cell\u2010blocks (n\u00a0=\u00a0284) were stained for claudin\u20104 and results compared with Ber\u2010Ep4. Cases included 172 metastatic malignancies (137 adenocarcinomas, 20 small cell lung tumours, eight metastatic melanoma, four squamous cell carcinoma, three urothelial cell carcinoma), 49 benign reactive cases and 63 mesotheliomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 49 benign effusions were negative. Only 1\/63 (1.6%) mesotheliomas was positive for claudin\u20104. Claudin\u20104 staining was positive in 131\/137 (95.6%) adenocarcinoma cases. Cases negative for claudin\u20104 included single cases of metastases from breast, colon, stomach, prostate, kidney and ovary. Claudin\u20104 outperformed Ber\u2010Ep4. Sensitivity (95.6% vs 85.4%), specificity (99.1% vs 86.6%), negative predictive value (94.9% vs 82.9%) and positive predictive value (99.2% vs 88.6%) were all higher for claudin\u20104 compared with Ber\u2010Ep4, respectively. Only two cases were claudin\u20104\u2212\/Ber\u2010Ep4+. Significantly (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0064) more cases of metastatic adenocarcinoma stained positive for claudin\u20104 (131\/137; 95.6%) than Ber\u2010Ep4 (117\/137; 86.2%). Claudin\u20104 staining was present in 15\/20 (75%) of neuroendocrine carcinomas, 3\/4 (75%) <jats:styled-content style=\"fixed-case\">squamous cell carcinoma<\/jats:styled-content> and 3\/3 (100%) <jats:styled-content style=\"fixed-case\">urothelial cell carcinoma<\/jats:styled-content>. All eight cases of melanoma were negative for both claudin\u20104 and Ber\u2010Ep4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Claudin\u20104 staining is a useful addition to <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> panels for effusions specimens with superior performance to Ber\u2010Ep4.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12765","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T13:07:40Z","timestamp":1565183260000},"page":"614-619","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Claudin\u20104 immunohistochemistry is a useful pan\u2010carcinoma marker for serous effusion specimens"],"prefix":"10.1111","volume":"30","author":[{"given":"Melissa","family":"Vojtek","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences Faculty of Health Queensland University of Technology  Brisbane Qld Australia"},{"name":"Sullivan and Nicolaides Pathology  Bowen Hills Qld Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Walsh","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences Faculty of Health Queensland University of Technology  Brisbane Qld Australia"},{"name":"Sullivan and Nicolaides Pathology  Bowen Hills Qld Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Papadimos","sequence":"additional","affiliation":[{"name":"Sullivan and Nicolaides Pathology  Bowen Hills Qld Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4596-2225","authenticated-orcid":false,"given":"Paul W.","family":"Shield","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences Faculty of Health Queensland University of Technology  Brisbane Qld Australia"},{"name":"Sullivan and Nicolaides Pathology  Bowen Hills Qld Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-186"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21392"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21380"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-007-0448-x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447008"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.028589"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00125480-200607000-00004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/112.1.75"},{"key":"e_1_2_8_10_1","volume-title":"The Art and Science of Cytopathology","author":"DeMay RM","year":"2012"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP0B3YJBXWXJII"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH726>3.0.CO;2-M"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12765","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cyt.12765","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.12765","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T06:39:05Z","timestamp":1694241545000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.12765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,9]]},"references-count":11,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/cyt.12765"],"URL":"https:\/\/doi.org\/10.1111\/cyt.12765","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,9]]},"assertion":[{"value":"2019-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}