{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:05:39Z","timestamp":1785679539981,"version":"3.56.0"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004099","name":"Ligue Contre le Cancer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Diffuse malignant peritoneal mesothelioma (<jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content>), represents 30% of all malignant mesothelioma, and is characterised by a difficult diagnosis and different presentations. Immunohistochemistry has improved the diagnostic sensitivity and specificity in the differential diagnosis between metastatic adenocarcinoma and malignant mesothelioma, and loss of <jats:styled-content style=\"fixed-case\">BRCA<\/jats:styled-content>\u20101\u2010associated protein 1 (<jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1) expression is correlated with <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 somatic or constitutional genetic defects. Furthermore, cyclin\u2010dependent kinase inhibitor 2A (<jats:italic><jats:styled-content style=\"fixed-case\">CDKN<\/jats:styled-content>2A<\/jats:italic>) is frequently lost in <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content>. In the present study, we assessed the value of integrating <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 in the panel of antibodies used for the diagnosis of <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> in cytological samples. Since p16 fluorescent in situ hybridisation (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) assay could constitute an additional useful adjunct, results of <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 immunostaining and p16 <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> assays have been compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010eight <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> patients and 71 peritoneal carcinomatosis patients were included. <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 immunohistochemical and <jats:italic><jats:styled-content style=\"fixed-case\">CDKN<\/jats:styled-content>2A <\/jats:italic><jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> techniques were performed on tissue specimens of <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> (n\u00a0=\u00a048) and peritoneal carcinomatosis (n\u00a0=\u00a071) then on cell\u2010block of <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> (n\u00a0=\u00a016), peritoneal carcinomatosis (n\u00a0=\u00a025) and peritoneal benign effusion (n\u00a0=\u00a05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Loss of <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 expression was observed in 56.3% of <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> while none of the peritoneal carcinoma specimens showed <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 loss of expression. <jats:italic><jats:styled-content style=\"fixed-case\">CDKN<\/jats:styled-content>2A<\/jats:italic> loss was observed in 34.9% <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> and 2.1% peritoneal carcinoma. Although <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 immunostaining was successful in 100% of cytological <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> samples, <jats:italic><jats:styled-content style=\"fixed-case\">CDKN<\/jats:styled-content>2A<\/jats:italic> deletion status could be obtained for 75% of <jats:styled-content style=\"fixed-case\">DMPM<\/jats:styled-content> cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>1 immunostaining represents an objective and reproducible diagnostic biomarker for peritoneal mesothelioma in effusion cytology specimens and should be preferred to <jats:italic><jats:styled-content style=\"fixed-case\">CDKN<\/jats:styled-content>2A <\/jats:italic><jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> analysis on these precious samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12788","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T10:05:07Z","timestamp":1573553107000},"page":"5-11","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The value of BRCA\u20101\u2010associated protein 1 expression and cyclin\u2010dependent kinase inhibitor 2A deletion to distinguish peritoneal malignant mesothelioma from peritoneal location of carcinoma in effusion cytology specimens"],"prefix":"10.1111","volume":"31","author":[{"given":"Wajd","family":"Althakfi","sequence":"first","affiliation":[{"name":"Department of Pathology King Saud University  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Gazzo","sequence":"additional","affiliation":[{"name":"Department of Hematology HCL Cancer Institute and Lyon 1 University  Lyon 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