{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:50:42Z","timestamp":1771462242452,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T00:00:00Z","timestamp":1585008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The development of a terminology system is essential to allow uniformity in reporting serous fluid specimens. An important topic to cover is the issue of specimen adequacy. In the present study, we aimed to evaluate whether there is a correlation between number of mesothelial cells and overall improved sensitivity and adequacy control of tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cases of negative pleural fluids with concomitant positive pleural biopsies were selected from two referral institutions, with observation of the number of mesothelial cells in 10 high\u2010power fields, comparing the results with a control group (cases with negative biopsies, ie, true negatives). Comparisons were conducted using the nonparametric Mann\u2010Whitney <jats:italic>U<\/jats:italic> test. Data were analysed for sensitivity and specificity derived from the receiver operating characteristics curve. For the choice of an optimal cut\u2010off of mesothelial cells, receiver operating curve analysis was constructed and the Youden index was calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 112 pleural effusions with paired pleural biopsies were studied. There was no difference in distributions of the number of mesothelial cells between cases with a positive biopsy (false negatives) and the control group (median\u00a0=\u00a039 vs median\u00a0=\u00a030, respectively, <jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a0.974). However, simple logistic regression found a cut\u2010off of 750 cells per 10 <jats:styled-content style=\"fixed-case\">high\u2010power fields<\/jats:styled-content> as an optimal number for improved sensitivity (72.7%), with fair discriminatory power.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Enumeration of mesothelial cells may improve the sensitivity of the cytological diagnosis of <jats:styled-content style=\"fixed-case\">malignant pleural effusion<\/jats:styled-content>, serving as an internal quality control for the test's overall accuracy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12808","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T07:20:30Z","timestamp":1581492030000},"page":"223-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Number of mesothelial cells as a measure of adequacy criteria for pleural effusions: A multi\u2010institutional study"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6757-1172","authenticated-orcid":false,"given":"Erika F.","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Department of Pathology Johns Hopkins Hospital  Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul C.","family":"Morris","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Hospital  Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinicius","family":"Calsavara","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Statistics A.C. Camargo Cancer Center  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0124-2437","authenticated-orcid":false,"given":"Ricardo G.","family":"Pastorello","sequence":"additional","affiliation":[{"name":"Department of Pathology A.C. Camargo Cancer Center  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9438-226X","authenticated-orcid":false,"given":"Mauro","family":"Saieg","sequence":"additional","affiliation":[{"name":"Department of Pathology A.C. Camargo Cancer Center  S\u00e3o Paulo Brazil"},{"name":"Department of Pathology Santa Casa Medical School  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,24]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Pleural Diseases","author":"Light RW","year":"2013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1403503"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477148"},{"issue":"7","key":"e_1_2_8_5_1","first-page":"1169","article-title":"Molecular profiling of malignant pleural effusion in metastatic non\u2010small\u2010cell lung carcinoma the effect of preanalytical factors","volume":"14","author":"Carter J","year":"2017","journal-title":"Ann Am Thorac Soc"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21518"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0091-SA"},{"key":"e_1_2_8_8_1","first-page":"1","article-title":"Contemporary approach to the diagnosis of malignant pleural effusion","author":"Kaul V","year":"2019","journal-title":"Ann Am Thorac Soc"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2010.136978"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)60212-2"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arbres.2013.11.007"},{"key":"e_1_2_8_12_1","first-page":"1","article-title":"Announcement: the International system for reporting serous fluid cytopathology","author":"Chandra A","year":"2019","journal-title":"Acta Cytol"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000493336"},{"issue":"4","key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1183\/09031936.93.02040366","article-title":"Pleural metastatic tumours and effusions. 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