{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T08:46:34Z","timestamp":1766133994680,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,9,5]],"date-time":"2020-09-05T00:00:00Z","timestamp":1599264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The American Society of Clinical Oncology (ASCO)\u2010endorsed College of American Pathologists guideline recommends high\u2010risk human papillomavirus (HPV) testing for metastatic squamous cell carcinoma (SCC) of lymph nodes level II\/III of unknown primary. Herein, the performance of HPV\u2010RNA in situ hybridisation (ISH) in detection of HPV\u2010related SCC is evaluated implementing the ASCO guideline recommendations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighty head and neck (HN) SCC fine needle aspirations, which utilized HPV\u2010RNA ISH\/P16, were evaluated at Johns Hopkins Hospital (2015\u20102018) to investigate their performance and concordance with histology. The results were compared to a prior study of 59 HNSCCs, which HPV\u2010DNA ISH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 80 reviewed fine needle aspirations, 65 (50 male, 15 female) were included. The mean age was 63.2\u00a0\u00b1\u00a014.0\u00a0years. The most common site was neck lymph nodes (47, 72.3%). Fifty\u2010five cases (84.6%) were accompanied by concurrent core biopsy, and 48 cases (59.4%) had surgical follow\u2010ups. HPV\u2010RNA ISH was positive in 44 (67.7%), and P16 was strongly positive in 46 (70.8%). The HPV\u2010RNA ISH\/ P16 concordance rate was 92.3% on cytology material. The cytology\/surgical concordance rate for HPV\u2010RNA ISH was 88.9% (16\/18). There was a discordance between the results in five cases (7.7%; HPV\u2010RNA ISH\u2010\/P16+).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HPV\u2010RNA ISH is a robust and reliable method for detecting HPV\u2010related HNSCC on cytology material showing concordance rate of 92.3% between HPV\u2010RNA ISH and P16, which is a sensitive but non\u2010specific marker. Compared to HPV\u2010DNA ISH, HPV\u2010RNA ISH reproducibly identifies HPV\u2010related HNSCC with fewer discrepancies between cytology and histology. The findings of this study are in agreement with the ASCO recommendations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12896","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T21:00:35Z","timestamp":1596229235000},"page":"547-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Diagnostic utility of high\u2010risk human papillomavirus mRNA in situ hybridisation in squamous cell carcinoma of unknown primary in the head and neck and implementing American Society of Clinical Oncology guideline recommendations"],"prefix":"10.1111","volume":"31","author":[{"given":"Parnaz","family":"Daneshpajouhnejad","sequence":"first","affiliation":[{"name":"Department of Pathology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James Adam","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical College of Wisconsin  Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3273-4802","authenticated-orcid":false,"given":"Zahra","family":"Maleki","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,5]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1200\/JCO.18.00684"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1002\/lary.20638"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_1","DOI":"10.1001\/archoto.2009.145"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_1","DOI":"10.1200\/JCO.20.00275"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1002\/dc.23668"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_1","DOI":"10.1002\/hed.24603"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_1","DOI":"10.1016\/j.jasc.2015.06.002"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_1","DOI":"10.1001\/jamaoncol.2016.3067"},{"key":"e_1_2_9_10_1","first-page":"344","article-title":"HPV\u2010related squamous cell carcinoma of the head and neck: an update on testing in routine pathology practice. 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