{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:38:10Z","timestamp":1787315890592,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The goal of this study was to evaluate the performance of p16 staining in cell\u2010blocks vs tissue specimens as a surrogate marker for human papillomavirus (HPV) status in oropharyngeal squamous cell carcinomas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Head and neck squamous cell carcinoma cases presenting as a neck mass with a p16 result on cytology and corresponding tissue specimens (1 January 2014 to 30 June 1920) were included in the study. The following were assessed from cell\u2010block material: number of tumour clusters, percentage of tumour cells with p16 staining, and presence of staining in clusters vs single cells. Results were compared to tissue p16 status. Results of any other ancillary HPV testing were also noted.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Forty\u2010two head and neck squamous cell carcinoma neck metastases (35 oropharyngeal, five non\u2010oropharyngeal, and 2 unknown primaries) were identified. The p16 staining pattern in cell\u2010blocks was seen in single cells (27.6%), clusters (44.8%), or both (27.6%). The percentage of tumour cells staining for p16 in cell\u2010blocks was much lower than in corresponding tissue specimens. There were four false negatives and one\n                      <jats:italic>false positive<\/jats:italic>\n                      (concurrent HPV DNA polymerase chain reaction testing was positive in cytology and surgical material).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Compared to tissue, the cut\u2010off for p16 interpretation in cell\u2010blocks is substantially lower and staining may be present in single cells or clusters. In 96.9% of cases, any p16 staining in cell\u2010blocks correlated with positive p16 staining in surgical specimens. However, a negative or discrepant p16 result on cell\u2010block should prompt confirmatory HPV studies, as false negative p16 staining in cell\u2010blocks is high.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cyt.12911","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T15:59:12Z","timestamp":1599235152000},"page":"100-107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Correlation of p16 immunostaining in cell\u2010blocks with corresponding tissue specimens for squamous cell carcinomas of the oropharynx"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1090-9715","authenticated-orcid":false,"given":"Sharon J.","family":"Song","sequence":"first","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine University of Pennsylvania Health System  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6978-0242","authenticated-orcid":false,"given":"Jalal B.","family":"Jalaly","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine University of Pennsylvania Health System  Philadelphia PA 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