{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:23:27Z","timestamp":1784172207522,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the efficacy of high\u2010risk human papillomavirus (HR\u2010HPV) and DNA image cytometry (DNA\u2010ICM) status for identifying high\u2010grade cervical intraepithelial neoplasia or worse (\u2265CIN2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This cross\u2010sectional study was performed in women undergoing follow\u2010up procedure after a previous abnormal cervical cytology. Cervical cells were collected for HPV detection and DNA ploidy measurement. Biopsy samples were taken for histological confirmation. Sensitivity and specificity values for \u2265CIN2 detection with HR\u2010HPV and DNA\u2010ICM were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HR\u2010HPV was present in 74.5% of the women. The most frequent HPV infection was HPV 16, followed by HPV 31, 33 and 58. Aneuploidy was observed in 60.6% of all cases. Referral cytology revealed 78.0% sensitivity and 68.6% specificity for detecting a\u00a0\u2265CIN2 lesion. The HR\u2010HPV test alone showed 92.7% sensitivity, albeit it was not statistically different from DNA\u2010ICM (88.1%, <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.05). Positivity for HPV or DNA\u2010ICM resulted in 100% sensitivity. Higher specificity was observed for the combination of HR\u2010HPV and DNA\u2010ICM (88.6%), with no difference from DNA\u2010ICM alone (85.7%, <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DNA\u2010ICM or HR\u2010HPV positivity identified all cases of\u00a0\u2265CIN2 in women undergoing follow\u2010up procedure after a previous abnormal cervical cytology. Routine cervical cancer screening could be improved by the incorporation of DNA\u2010ICM as a complementary method to primary screening to identify which women need closer follow\u2010up.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12943","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T01:49:11Z","timestamp":1605923351000},"page":"180-186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["DNA ploidy measurement and human papillomavirus in abnormal cervical cytology"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1467-4877","authenticated-orcid":false,"given":"Ane Francyne","family":"Costa","sequence":"first","affiliation":[{"name":"Department of Clinical Analysis Federal University of Santa Catarina Florian\u00f3polis 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