{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:57:25Z","timestamp":1747184245542,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T00:00:00Z","timestamp":1618272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Cervical cancer screening by primary human papilloma virus detection and cytology is fraught with low specificity and variable sensitivity, respectively. Cytology\u2010histology correlation remains modest. Biomarkers associated with early genetic events in cervical squamous carcinogenesis and detectable in cytology material are likely to be relevant. Human telomerase RNA component (hTERC) gene overexpression and aneuploidy are promising candidates in view of their reported early and consistent association with cervical squamous oncogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analysed hTERC gene expression and chromosome 7 ploidy by fluorescent in\u2010situ hybridisation (FISH) in 50 women with cytological precursor squamous intraepithelial lesions and available histology outcomes. Results were expressed as percentages of cells showing \u22653 signals, mean signals\/nucleus, and maximum amplitude across various cytology and histology categories. Proportions of positive cases were calculated from threshold values derived from 6 controls. Distribution of above indices with respect to \u2265cervical intraepithelial neoplasia 2 (CIN2) was explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For both genetic aberrations, there was significant positive correlation (for all indices) between the proportion of positive cases and worsening cytological and histological outcomes (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05), with significant intergroup differences (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). High\u2010grade lesions (\u2265CIN2) had significantly higher results compared to &lt;CIN2 lesions (<jats:italic>P<\/jats:italic>\u00a0\u2264\u00a0.001). In five discordant cases with \u2265CIN2 under\u2010 or overdiagnosed on cytology, FISH supported the histological diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HTERC gene amplification and chromosome 7 ploidy showed positive association with cervical squamous carcinogenesis and could be relevant in settings of discrepant cytology\u2010histology correlation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12978","type":"journal-article","created":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T02:24:30Z","timestamp":1618367070000},"page":"631-639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Human telomerase RNA component (hTERC) gene expression and chromosome 7 ploidy correlate positively with histological grade of cervical intraepithelial neoplasia"],"prefix":"10.1111","volume":"32","author":[{"given":"Divya","family":"Aggarwal","sequence":"first","affiliation":[{"name":"Department of Pathology University College of Medical Sciences &amp; Guru Teg Bahadur Hospital University of Delhi Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4102-2439","authenticated-orcid":false,"given":"Neelam","family":"Wadhwa","sequence":"additional","affiliation":[{"name":"Department of Pathology University College of Medical Sciences &amp; Guru Teg Bahadur Hospital University of Delhi Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanvi","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Pathology University College of Medical Sciences &amp; Guru Teg Bahadur Hospital University of Delhi Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shalini","family":"Rajaram","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University College of Medical Sciences &amp; Guru Teg Bahadur Hospital University of Delhi Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Preeti","family":"Diwaker","sequence":"additional","affiliation":[{"name":"Department of Pathology University College of Medical Sciences &amp; Guru Teg Bahadur Hospital University of Delhi Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashutosh","family":"Halder","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology All India Institute of Medical Sciences Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manish","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology All India Institute of Medical Sciences Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiran","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Pathology University College of Medical Sciences &amp; Guru Teg Bahadur Hospital University of Delhi Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(19)30482-6"},{"key":"e_1_2_9_3_1","unstructured":"Three\u2010Year Report of Population Based Cancer Registries 2012\u20102014. Chapter 7: Comparison of cancer incidence and patterns of all population based cancer registries. National Cancer Registry Programme National Centre for Disease Informatics and Research http:\/\/ncdirindia.org\/NCRP\/ALL_NCRP_REPORTS\/PBCR_REPORT_2012_2014\/ALL_CONTENT\/PDF_Printed_Version\/Chapter7_Printed.pdf. 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