{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T09:16:11Z","timestamp":1720170971727},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Human immunodeficiency virus\u2010infected women have a high incidence of HPV infection, and HIV and HPV coinfection is associated with high incidence of cervical intraepithelial lesions and cervical cancer. This study investigated the ability to detect HIV mRNA in routine screening cervical liquid\u2010based cytology (LBC) samples and its correlation with HPV coinfection and cervical intraepithelial lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liquid\u2010based cytology samples from 80 HIV\u2010infected women under combined antiretroviral therapy (cART) were studied for detection of HIV and HPV mRNA using Aptima<jats:sup>\u00ae<\/jats:sup> tests and for cytology diagnosis according to the 2014 Bethesda System for Reporting Cervical Cytology. Peripheral blood (PB) HIV mRNAs were assessed by real\u2010time polymerase chain reaction (RT\u2010PCR). Statistical analysis used Fisher's exact or Chi\u2010square test to compare frequencies among groups and the Mann\u2010Whitney <jats:italic>U<\/jats:italic> test to compare continuous variables.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Human immunodeficiency virus mRNA was present in 21.3% of routine LBC samples in HIV\u2010infected women, 12.5% of which had no detectable PB viral load. Among 10 patients diagnosed with high\u2010grade squamous intraepithelial lesion (HSIL), 50% had detectable HIV viral load. The occurrence of HSIL vs low\u2010grade intraepithelial lesion\/negative intraepithelial lesion or malignancy in LBC samples was significantly higher in women with detectable HIV viral load (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.029).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Human immunodeficiency virus mRNA was present in routine LBC samples in HIV\u2010positive women under cART. Detection of HIV viral load in LBC is significantly associated with cervical HSIL. This suggests the relevance of HIV mRNA viral load assessment in routine LBC, to evaluate patients' infectious potential and monitor efficacy of the cART scheme.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12983","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T15:55:43Z","timestamp":1619711743000},"page":"640-645","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of HIV mRNA in routine liquid\u2010based cytology specimens of HIV\u2010infected women"],"prefix":"10.1111","volume":"32","author":[{"given":"Maria Jos\u00e9","family":"Brito","sequence":"first","affiliation":[{"name":"Department of Pathology Hospital Garcia de Orta Almada Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Quintas","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Hospital Garcia de Orta Almada 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