{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:36:56Z","timestamp":1765888616853,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T00:00:00Z","timestamp":1622419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Currently, it is thought that uterine cervix mucosal samples present a low risk of SARS\u2010CoV\u20102 exposure. So far, there is no evidence of SARS\u2010CoV\u20102 detection in Papanicolaou (Pap) smears. Nevertheless, clinicians could be exposed unaware to the coronavirus while performing and handling a Pap smear. We aimed to retrospectively evaluate the presence of SARS\u2010CoV\u20102 RNA in cervical liquid\u2010based cytology (LBC) samples in women who tested positive for a nasopharyngeal COVID\u201019 PCR test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>From our laboratory database, we identified patients with data on a cervical cancer screening LBC sample paired with a positive nasopharyngeal COVID\u201019 PCR test. Relevant LBC samples taken within an incubation period of 14\u00a0days and post\u2010onset RNA shedding interval of 25\u00a0days were subsequently tested for SARS\u2010CoV\u20102 RNA using RT\u2010PCR tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study group consisted of 102 women. Of those, 23 LBC samples were tested. SARS\u2010CoV\u20102 RNA was detected in one LBC sample from a 26\u2010year\u2010old asymptomatic woman taken six days before reporting headaches and knee arthralgia with a positive nasopharyngeal SARS\u2010CoV\u20102 RT\u2010PCR test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>It is possible to detect SARS\u2010CoV\u20102 RNA in cervical LBC samples at an early asymptomatic stage of COVID\u201019. In general, this finding is infrequent in asymptomatic women who tested SARS\u2010CoV\u20102 positive within an incubation of 14\u00a0days and a post\u2010onset RNA shedding period of 25\u00a0days. We fully support the current thinking that cervical LBC samples from asymptomatic women pose a low risk of SARS\u2010CoV\u20102 exposure and can be handled in the frame of good microbiological practice and procedures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.12995","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T15:55:43Z","timestamp":1621958143000},"page":"766-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["SARS\u2010CoV\u20102 RNA may rarely be present in a uterine cervix LBC sample at the asymptomatic early stage of COVID 19 disease"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4038-5641","authenticated-orcid":false,"given":"Ondrej","family":"Ondi\u010d","sequence":"first","affiliation":[{"name":"Department of Pathology Medical Faculty Charles University and Charles University Hospital Pilsen Pilsen Czech Republic"},{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate\u0159ina","family":"\u010cern\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical Faculty Charles University and Charles University Hospital Pilsen Pilsen Czech Republic"},{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Kinkorov\u00e1\u2010Lu\u0148\u00e1\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"N\u011bmcov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical Faculty Charles University and Charles University Hospital Pilsen Pilsen Czech Republic"},{"name":"Bioptick\u00e1 Laborato\u0159, s.r.o. Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u0159ivoj","family":"Mejchar","sequence":"additional","affiliation":[{"name":"Private Gynaecologist Office Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Chytra","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Medical Faculty Charles University and Charles University Hospital Pilsen Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u0159\u00ed","family":"Bouda","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Medical Faculty Charles University and Charles University Hospital Pilsen Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22373"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22354"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12953"},{"key":"e_1_2_9_5_1","unstructured":"World Health Organisation (WHO).Laboratory testing for coronavirus disease 2019 (COVID\u201019) in suspected human cases.https:\/\/www.who.int\/publications\u2010detail\/laboratory\u2010testing\u2010for\u20102019\u2010novel\u2010coronavirus\u2010in\u2010suspected\u2010humancases\u201020200117. 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