{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T22:45:50Z","timestamp":1775083550720,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>HIV\u2010positive men who have sex with men (MSM) are a vulnerable group for anal cancer (AC), a cancer with a well\u2010described precursor lesion, which can be detected early in screening programs using anal liquid\u2010based cytology (aLBC). We aim to compare two aLBC sample collection devices: cytology brush (CB) and Dacron swab (DS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Retrospective analysis of two consecutive study periods, the first using CB and the second DS. Participants underwent an aLBC, a human papillomavirus (HPV) DNA test and a high\u2010resolution anoscopy (HRA), and a biopsy was performed for suspicious lesions. The sensitivity and specificity of aLBC, area under the receiver operating characteristic (ROC) curve (AUC), and concordance between cytology and HRA were assessed using Cohen\u2019s kappa coefficient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 239 participants were enrolled (CB group, 120; DS group, 119). aLBC was benign in 46% of samples, and high\u2010grade squamous intraepithelial lesion (HSIL) was detected in 11.7%. Prevalence of biopsy\u2010proven HSIL was 15.3%. No differences in cytological and histological results were observed between the groups. aLBC\u2010HRA concordance was weak for benign results (CB group, k\u00a0=\u00a00.309; DS group, k\u00a0=\u00a00.350) as well as for HSIL (k\u00a0=\u00a00.321 and 0.387, respectively). Sensitivity and specificity were 100% and 51.4%, respectively, in the CB group and 88% and 54.3% in the DS group (AUC\u00a0=\u00a00.711 and 0.759, respectively,<jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a0.514). Representation of the transformation zone (TZ) was adequate in 83.3% of samples in the CB group and 50.4% in the DS group (<jats:italic>P<\/jats:italic>\u2010value &lt;.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest that both devices had similar accuracy to detect anal HSIL, although samples collected with CB are more likely to have an adequate TZ representation, the presence of which could be an indicator of sample quality.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13013","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T15:55:54Z","timestamp":1621958154000},"page":"646-653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of two sample collection devices for anal cytology in HIV\u2010positive men who have sex with men: Cytology brush and Dacron swab"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4660-534X","authenticated-orcid":false,"given":"Ana C.","family":"Silva\u2010Klug","sequence":"first","affiliation":[{"name":"HIV and STD Unit (Infectious Disease Service) Bellvitge University Hospital\/Bellvitge Biomedical Research Institute (IDIBELL) L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Saumoy","sequence":"additional","affiliation":[{"name":"HIV and STD Unit (Infectious Disease Service) Bellvitge University Hospital\/Bellvitge Biomedical Research Institute (IDIBELL) L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"Baixeras","sequence":"additional","affiliation":[{"name":"Pathology Unit Bellvitge University Hospital\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loris","family":"Trenti","sequence":"additional","affiliation":[{"name":"Colorectal Unit, General and Digestive Surgery Department Bellvitge University Hospital\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Catala","sequence":"additional","affiliation":[{"name":"Pathology Unit Bellvitge University Hospital\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"August","family":"Vidal","sequence":"additional","affiliation":[{"name":"Pathology Unit Bellvitge University Hospital\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Montserrat","family":"Torres","sequence":"additional","affiliation":[{"name":"Infection and Cancer Laboratory Cancer Epidemiology Research Program Catalan Institute of Oncology (ICO)\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laia","family":"Alemany","sequence":"additional","affiliation":[{"name":"Infection and Cancer Laboratory Cancer Epidemiology Research Program Catalan Institute of Oncology (ICO)\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"},{"name":"CIBERen Epidemiolog\u00eda y Salud P\u00fablica CIBERESP Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n","family":"Videla","sequence":"additional","affiliation":[{"name":"Clinical Research Support Unit Clinical Pharmacology Department Bellvitge University Hospital\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"De San Jose","sequence":"additional","affiliation":[{"name":"Infection and Cancer Laboratory Cancer Epidemiology Research Program Catalan Institute of Oncology (ICO)\/IDIBELL L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Podzamczer","sequence":"additional","affiliation":[{"name":"HIV and STD Unit (Infectious Disease Service) Bellvitge University Hospital\/Bellvitge Biomedical Research Institute (IDIBELL) L'Hospitalet de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70080-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir1012"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000000254"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28963"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30653-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128165"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21702"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e328359f255"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078291"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000000160"},{"key":"e_1_2_8_12_1","first-page":"ciz831","article-title":"Routine 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A prospective cohort study","author":"Revollo B","year":"2019","journal-title":"Clin Infect Dis"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4240\/wjgs.v8.i1.41"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70215-2"},{"issue":"2","key":"e_1_2_8_15_1","first-page":"122","article-title":"Human papillomavirus, anal cancer, and screening considerations among HIV\u2010infected individuals","volume":"15","author":"Cachay ER","year":"2013","journal-title":"AIDS Rev"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22018"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1071\/SH11178"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27431"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188851"},{"key":"e_1_2_8_20_1","first-page":"ciz144","article-title":"High\u2010risk human papilloma virus testing improves diagnostic performance to predict moderate\u2010to\u2010high grade anal intraepithelial neoplasia in HIV\u2010infected men who have sex with men in low\u2010to\u2010absent cytological abnormalities","author":"Viciana P","year":"2019","journal-title":"Clin Infect Dis"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-11074-5"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21730"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0b013e31825c31dd"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)02353-3"},{"issue":"4","key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/dc.21201","article-title":"Anal cytology in patients with AIDS","volume":"38","author":"Carole EE","year":"2010","journal-title":"Diagn Cytopathol"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jana.2011.08.006"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000447"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12299"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/CEJ.0000000000000321"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11121942"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13013","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cyt.13013","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T14:32:18Z","timestamp":1725114738000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.13013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,28]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/cyt.13013"],"URL":"https:\/\/doi.org\/10.1111\/cyt.13013","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,28]]},"assertion":[{"value":"2021-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}