{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T09:56:44Z","timestamp":1752227804949,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T00:00:00Z","timestamp":1637884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Cytological diagnosis of pancreatic specimens obtained by endoscopic ultrasound\u2010guided fine needle aspiration (EUS\u2010FNA) is often challenging because of the small sample size or well\u2010differentiated adenocarcinoma with weak cytological atypia. Therefore, the sensitivity and specificity of cytological diagnosis for pancreatic cancer should be improved. Hence, we aimed to clarify the utility of cytological scoring to distinguish malignant from benign lesions for cytological diagnosis of pancreatic EUS\u2010FNA specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seven reviewers, including four cytotechnologists and three medical doctors, evaluated 20\u00a0morphological indices in pancreatic specimens obtained by EUS\u2010FNA (malignant, <jats:italic>n<\/jats:italic>\u00a0=\u00a0111; benign, <jats:italic>n<\/jats:italic>\u00a0=\u00a031). Statistical analyses were performed using Fisher's exact test, logistic regression analysis, the area under the receiver operating characteristic curve, and Youden index.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 20 indices, there was a high incidence rate (&gt;40%) of the following 13 indices in malignant cases: irregular structure, hyperchromatic nucleus, irregular cell polarity, unclear cell boundaries, nuclear membrane thickening, anisonucleosis, overlapping, irregular nuclei, high nuclear\u2010cytoplasmic ratio, binding decline, the simultaneous appearance of malignant and benign cells, enlarged nucleoli, and background necrosis. When we diagnosed pancreatic specimens using these 13 cytological indices, the cut\u2010off value of 8\/9\u00a0showed the highest Youden index (0.950) as well as high sensitivity and specificity in distinguishing malignant from benign specimens (98% and 97%, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Thirteen cytological indices showed high sensitivity and specificity in differentiating malignant and benign lesions using pancreatic EUS\u2010FNA samples. All 13 indices were important for diagnosing malignancy in the pancreatic cytology smear of EUS\u2010FNA. Further validation studies are required.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13076","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T16:50:33Z","timestamp":1636563033000},"page":"206-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cytological scoring for pancreatic specimens obtained by endoscopic ultrasound\u2010guided fine needle aspiration"],"prefix":"10.1111","volume":"33","author":[{"given":"Yuri","family":"Kiso","sequence":"first","affiliation":[{"name":"Department of Pathology Tokyo Metropolitan Geriatric Hospital Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3235-655X","authenticated-orcid":false,"given":"Yoko","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Pathology Tokyo Metropolitan Geriatric Hospital Tokyo Japan"},{"name":"Department of Oncology Pathology 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