{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T11:37:44Z","timestamp":1759837064470,"version":"3.40.5"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T00:00:00Z","timestamp":1639958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>This study aimed to determine the causes of disruption of the three\u2010dimensional architecture of endometrial glands prepared using BD SurePath\u2122 liquid\u2010based cytology (SP\u2010LBC) reagents. One sample preparation method for endometrial cytology is presented in which this three\u2010dimensional architecture can be retained.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>SP\u2010LBC specimens were prepared by the following three methods: (1) using the BD PrepMate<jats:sup>TM<\/jats:sup> (PrepMate) System after cellular fixation for 1\u20106\u00a0h (method A); (2) without using the PrepMate System after cellular fixation for 1\u20106\u00a0h (method B); and (3) using the PrepMate System after cellular fixation for at least 18\u00a0h (method C). Size and numbers of endometrial cell clusters and numbers of solitary scattered cells were then evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly higher numbers of cell clusters with a major axis of 200\u00a0\u03bcm or more were yielded by method C (71.3\u00a0\u00b1\u00a057.2) than methods A (9.3\u00a0\u00b1\u00a05.9, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) or B (44.3\u00a0\u00b1\u00a028.8, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Method B yielded significantly higher numbers of cell clusters than method A (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Method A (132.2\u00a0\u00b1\u00a0107.7, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) yielded significantly higher numbers of solitary scattered cells than methods B (29.1\u00a0\u00b1\u00a014.8) and C (35.7\u00a0\u00b1\u00a023.3). No significant difference in solitary cell numbers was found between methods B and C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Retention of endometrial glandular architecture is rendered possible by allowing sample fixation times of 18\u00a0h or more when preparing specimens using the PrepMate System.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13087","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T11:54:10Z","timestamp":1639050850000},"page":"357-361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A study on preserving endometrial glandular architecture during preparation using BD SurePath\u2122 liquid\u2010based cytology reagents: Cellular fixation with preservative fluid requires at least 18\u00a0h"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2970-3944","authenticated-orcid":false,"given":"Takeshi","family":"Nishikawa","sequence":"first","affiliation":[{"name":"Department of Pathology Nara Medical University Hospital  Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisae","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Pathology Nara Medical University Hospital  Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mao","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Pathology Nara Medical University Hospital  Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigenobu","family":"Tatsumi","sequence":"additional","affiliation":[{"name":"Department of Pathology Nara Medical University Hospital  Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuma","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Yamatotakada Municipal Hospital  Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiho","family":"Ohbayashi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University School of Medicine  Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8557-9207","authenticated-orcid":false,"given":"Tadao K.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Cancer Education and Research Center Osaka University Graduate School of Medicine  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5733-906X","authenticated-orcid":false,"given":"Yoshiaki","family":"Norimatsu","sequence":"additional","affiliation":[{"name":"Departments of Medical Technology Faculty of Health Sciences Ehime Prefectural University of Health Sciences  Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20599"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21734"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-3154-0.00026-0"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23916"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000502108"},{"key":"e_1_2_12_7_1","first-page":"25","article-title":"Establishment of the new sample preparing technique in endometrial fluid specimen cytodiagnosis (liquid\u2010based cytology, LBC) by SurePath method to preserve the original cell cluster construction: comparative investigation among the previous automatic method, the manual one, and our improved version of manual one","volume":"23","author":"Toda S","year":"2014","journal-title":"Niigata\u2010ken Koseiren Med J"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000336258"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.01.022"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/33.8.3894502"},{"key":"e_1_2_12_11_1","first-page":"122","volume-title":"Histochemistry, Theoretical and Applied","author":"Everson Pearse AG","year":"1985"},{"key":"e_1_2_12_12_1","unstructured":"BD SurePathTM Preservative Fluid Material Safety Data Sheet. March 1 2011.https:\/\/www.rmlonline.com\/images\/data\/attachments\/0000\/1125\/surepath\u201003\u201001\u201011\u2010msds.pdfAccessed October 21 2021."},{"key":"e_1_2_12_13_1","first-page":"475","article-title":"Effect of preservative solutions of BD liquid\u2010based cytology on blood","volume":"64","author":"Kawanishi N","year":"2015","journal-title":"Jpn J Med Tech"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13087","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cyt.13087","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T18:28:11Z","timestamp":1692988091000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.13087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,20]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1111\/cyt.13087"],"URL":"https:\/\/doi.org\/10.1111\/cyt.13087","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"type":"print","value":"0956-5507"},{"type":"electronic","value":"1365-2303"}],"subject":[],"published":{"date-parts":[[2021,12,20]]},"assertion":[{"value":"2021-10-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}