{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:18:29Z","timestamp":1790050709830,"version":"4.0.1"},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diagnostic accuracy of fine needle aspiration cytology for lymphomas has markedly improved when used in conjunction with flow cytometry (FCM), especially to differentiate reactive lymph node aspirates from low\u2010grade lymphomas, a challenge for cytopathologists. Lymphoma diagnosis by FCM depends on detection of immunophenotypic B\u2010 and T\u2010cell outliers, also present in benign lymph nodes albeit in subsets of cells. These can mimic heterogenous populations obtained from lymphomas partially involving lymph nodes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the present study, FCM of fine needle aspiration\/scrape material from 30 cases of benign lymph nodes was evaluated for the distribution of lymphoid populations and the presence and percentages of immunophenotypic outliers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The majority of the samples were cervical nodes with all cases showing T\u2010cell predominance. CD5\/CD19 co\u2010expression was seen in 23.94\u00a0\u00b1\u00a09.51% (20 cases) and CD10\/CD19 co\u2010expression in 3.2%\u201320.2% (8 cases) of B\u2010cell events. None of these cases showed light chain restriction. Loss of CD7 (2%\u201028.4%) and CD5 (12.7%) expression was seen in 17 cases and 1 case, respectively, of T\u2010cell events.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Immunophenotypic outliers suggestive of lymphomas could be seen in non\u2010lymphomatous lymph node aspirates in a subset of the cell population. However, they could be defined as benign due to absence of light chain restriction and normal CD4:CD8 ratios in all but one case, which was confirmed to be benign after clinical correlation and excisional biopsy. Thus, multiparameter FCM along with clinicoradiological correlation can prevent overdiagnosis of lymphomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13098","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T05:59:08Z","timestamp":1642053548000},"page":"505-513","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Flow cytometry in reactive lymph node aspirates: Evaluation of distribution of populations with immunophenotypic outliers"],"prefix":"10.1111","volume":"33","author":[{"given":"Priyanka","family":"Goel","sequence":"first","affiliation":[{"name":"Department of Pathology Shri Guru Ram Rai Institute of Medical and Health Sciences  Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seema","family":"Acharya","sequence":"additional","affiliation":[{"name":"Department of Pathology Shri Guru Ram Rai Institute of Medical and Health Sciences  Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4630-016X","authenticated-orcid":false,"given":"Sana","family":"Ahuja","sequence":"additional","affiliation":[{"name":"Department of Pathology Shri Guru Ram Rai Institute of Medical and Health Sciences  Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/MN6J-4NJY-C5CG-1PLH"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20108"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-2870.172428"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000448679"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/QF6N-VAQW-N74H-4JE2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.1999.14.4.393"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000448799"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22038"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204687"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(99)01476-0"},{"issue":"6","key":"e_1_2_9_12_1","first-page":"539","article-title":"CD10 expression in follicular lymphoma and large cell lymphoma is different from that of reactive lymph node follicles","volume":"122","author":"Almasri NM","year":"1998","journal-title":"Arch Pathol Lab Med"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13098","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cyt.13098","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T20:47:11Z","timestamp":1692823631000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.13098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,26]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/cyt.13098"],"URL":"https:\/\/doi.org\/10.1111\/cyt.13098","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,26]]},"assertion":[{"value":"2021-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}