{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T16:29:37Z","timestamp":1748881777089,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T00:00:00Z","timestamp":1649289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Evaluate the performance of different DNA image cytometry (DNA\u2010ICM) ploidy parameters in the categorisation of DNA\u2010ICM results and identification of high\u2010grade cervical intraepithelial neoplasia or worse (\u2265 CIN2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cervical samples from 232 women were collected for DNA\u2010ICM analysis and biopsy confirmation. Five DNA parameters were used to define DNA aneuploidy: number of cells with exceeding events (EE) over 2.5cEE, 4cEE, 5cEE and 9cEE, and aneuploid stemlines. DNA\u2010ICM results were categorised as normal, suspicious, and abnormal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For individual DNA ploidy parameters, sensitivity values for 50 cells with 2.5cEE, 45 cells with 4cEE, 1 cell with 9cEE and aneuploid stemline were 72.95%. 54.1%, 69.67% and 54.1%, while specificity values were 80.0%, 90.0%, 89.09% and 95.45%, respectively. For the 5cEE parameter, the sensitivity values for 1, 2, 3, 4 and 5 cells were 93.44%, 85.25%, 81.97%, 77.87% and 75.41%, while specificity values were 46.36%, 63.64%, 74.55%, 76.36% and 80.91%, respectively. For categorised DNA\u2010ICM results, a suspicious result showed superior sensitivity than an abnormal result (87.70% vs 82.79%, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.031), but lower specificity (54.55% vs 75.45%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Both types of DNA\u2010ICM result were statistically significantly different from a normal result (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>For prognostic purposes, 1 cell with 9cEE, 45 cells with 4cEE and aneuploid stemline are the best parameters with which to categorise an abnormal DNA\u2010ICM result, followed by 50 cells with 2.5cEE and 4 cells with 5cEE. For screening purposes, 10 cells with 2.5cEE, 10 cells with 4cEE, and 2 cells with 5cEE are suitable parameters with which to categorise a suspicious DNA\u2010ICM result.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13121","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T13:34:46Z","timestamp":1648388086000},"page":"563-571","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["DNA image cytometry parameters to identify high\u2010grade cervical lesions"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1467-4877","authenticated-orcid":false,"given":"Ane Francyne","family":"Costa","sequence":"first","affiliation":[{"name":"Department of Clinical Analysis Federal University of Santa Catarina  Florian\u00f3polis 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