{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:45:56Z","timestamp":1784162756488,"version":"3.55.0"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T00:00:00Z","timestamp":1677542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>For patients with endometrial cancer, the <jats:italic>POLE<\/jats:italic> (polymerase epsilon) mutation (<jats:italic>POLE<\/jats:italic>mut)\u2010subtype, one of four molecular\u2010analysis\u2010based categories in the Cancer Genome Atlas (TCGA), has the best prognosis. The following histological characteristics are typically observed in endometroid carcinoma cases with the <jats:italic>POLE<\/jats:italic>mut\u2010subtype: (1) the presence of tumour giant cells, (2) numerous tumour\u2010infiltrating lymphocytes (TILs) and\/or peri\u2010tumoral lymphocytes, and (3) a high grade. However, in the context of cytology, the morphological characteristics of this subtype remain unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DNA extracted from formalin\u2010fixed paraffin\u2010embedded (FFPE) tissues was subjected to next\u2010generation sequencing analysis and categorised according to the TCGA classifications. Genomic mutation, tumour mutation burden (TMB), and microsatellite instability were also assessed. Cytological specimens of resected uteri obtained using the Papanicolaou method were histologically separated into three types.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seven out of 112 patients (6%) with endometrial cancer were diagnosed with the <jats:italic>POLE<\/jats:italic>mut\u2010subtype between January 2019 and August 2021. Tumour giant cells were observed in three cases (43%) on histology and cytology. TIL and\/or peritumoral lymphocytes with inflammatory cells were detected in five cases (71%) on histology and three cases (43%) on cytology. Cases in which these three characteristics were observed on both cytology and histology may have belonged to the <jats:italic>POLE<\/jats:italic>mut\u2010subtype. There were no cases in which these characteristics were absent on histology but present on cytology. TMB tended to be higher in cases when the three characteristics were observed in both cytological and histological findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Preoperative endometrial cytology highlighted the characteristics of the <jats:italic>POLE<\/jats:italic>mut\u2010subtype in the histological analysis of resected uterine specimens and has the potential to play an important role in treatment decisions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13215","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T08:28:41Z","timestamp":1675326521000},"page":"211-218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cytopathological features associated with <i>POLE<\/i> mutation in endometrial cancer"],"prefix":"10.1111","volume":"34","author":[{"given":"Shintaro","family":"Yanazume","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Iwakiri","sequence":"additional","affiliation":[{"name":"Department of Pathology Kagoshima University Hospital  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Graduate School of Medical and Dental Sciences Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikumi","family":"Kitazono","sequence":"additional","affiliation":[{"name":"Department of Pathology Kagoshima University Hospital  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiaki","family":"Akahane","sequence":"additional","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  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