{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:58:04Z","timestamp":1747184284876,"version":"3.40.5"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the sensitivity and specificity of DNA ploidy with cytology, human papillomavirus (HPV) testing and colposcopy in diagnosis of high\u2010grade cervical intraepithelial neoplasia (CIN) and to assess the role of aneuploidy in cervical lesions with the worst prognosis. A prospective observational cohort study was conducted on 254 women with altered colpocytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Colposcopy, biopsy, DNA\u2010ICM and HPV examinations were applied to cervical cytological and histological samples. Participants were evaluated every 6\u2009months and divided into two groups: \u2018Harm\u2019 and \u2018No\u2010harm\u2019. Logistic regression and multivariate COX model were used to identify independent risk factors for diagnosis and prognosis of high\u2010grade CIN, and ROC curve to assess the sensitivity and specificity of methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Variables \u2018age greater than or equal to 30\u2009years\u2019, \u2018lesion size greater than 20%\u2019, \u2018aneuploidy\u2019 and \u2018HPV 16\u2019 were associated with diagnosis of high\u2010grade CIN and \u2018aneuploidy\u2019 and \u2018women living with HIV\u2019, with a worse prognosis. Agreement for colposcopy was good, with a sensitivity of 79.3% and specificity of 94.4%; DNA\u2010ICM and cytology were moderate, with sensitivity of 74.6% and 72.3% and specificity of 85.3% and 76.1%, respectively. High\u2010risk HPV and HPV 16 tests were weak, with sensitivity of 75.0% and 43.75% and specificity of 50.0% and 88.64%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In relation to high\u2010grade CIN diagnosis, DNA\u2010ICM presented similar sensitivity and specificity to cytology and high\u2010risk HPV test when associated with HPV 16. Regarding prognosis, this research certifies that aneuploidy is considered a predictor of more severe cervical injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13320","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:47:47Z","timestamp":1698126467000},"page":"122-130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of <scp>DNA<\/scp> ploidy in diagnosis and prognosis of high\u2010grade cervical intraepithelial neoplasia: A prospective cohort study"],"prefix":"10.1111","volume":"35","author":[{"given":"Adriane","family":"Pogere","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Professor Polydoro Ernani of Sao Thiago University Hospital of the Federal University of Santa Catarina, Florian\u00f3polis  Florian\u00f3polis 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