{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T15:34:56Z","timestamp":1773848096125,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T00:00:00Z","timestamp":1724112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","award":["51956.616.19834"],"award-info":[{"award-number":["51956.616.19834"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oral cancer screening requires analysis capable of detecting changes preceding the clinical manifestation. Oral cytopathology studies as an oral cancer screening have shown promising results. This study aims to evaluate the use of cytopathology in a quali\u2010quantitative analysis using Papanicoloau staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four experimental groups were evaluated: control (CG), exposed to carcinogens (EG), potentially malignant disorder with and without epithelial dysplasia (D\u2010OPMDG and ND\u2010OPMDG) and oral squamous cell carcinoma (OSCCG). Oral smears were collected using a Cytobrush\u00ae and conventional exfoliative cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Oral Papanicolaou smears from 143 individuals were analysed in 8100 images. Qualitatively, non\u2010lesional groups exhibited minimal suspected cases (20% in CG and 5% in EG), in the OPMD groups the ability to identify altered cells was low (40% in D\u2010OPMDG and 0% in ND\u2010OPMDG). Conversely, a notable\u00a0100% accuracy was achieved in the OSCCG. Quantitatively, a higher percentage of anucleated and a lower percentage of intermediate cells were observed in the OPMDG; a higher proportion of parabasal\/suspicious cells was observed in OSCCG. The optimal threshold for improved accuracy in detecting suspected malignancies occurs when the count of parabasal\/suspicious cells exceeds 8\/100 cells examined. Cytomorphometric analysis revealed a higher nucleus\/cytoplasm ratio (N\/C) in OSCCG, with a best cutoff point indicating enhanced accuracy in discerning suspected malignancies when exceeding 17% of cells exhibiting this elevated ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is possible to suggest updates in cytomorphometric and quantitative analysis in the modified Bethesda system for the oral cavity including objective criteria in the risk classification, therefore improving oral cancer screening.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13427","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T02:35:58Z","timestamp":1724294158000},"page":"41-49","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Oral cancer screening using cytopathology: An advanced proposal with the Bethesda classification"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7905-0737","authenticated-orcid":false,"given":"Tatiana Wannmacher","family":"Lepper","sequence":"first","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Ferreira Prigol","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-7043","authenticated-orcid":false,"given":"Ana Laura","family":"Ferrares Espinosa","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor Cavalcante","family":"Guedes","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maikel","family":"Maciel R\u00f6nnau","sequence":"additional","affiliation":[{"name":"Informatics Institute \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0764-8999","authenticated-orcid":false,"given":"Nat\u00e1lia Batista","family":"Daroit","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex Nogueira","family":"Haas","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Visioli","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Menezes de Oliveira Neto","sequence":"additional","affiliation":[{"name":"Informatics Institute \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9307-1980","authenticated-orcid":false,"given":"Pantelis Varvaki","family":"Rados","sequence":"additional","affiliation":[{"name":"Dental School \u2013 Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32871"},{"key":"e_1_2_10_3_1","unstructured":"World Health Organization (WHO).World Report on Violence and Health. 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