{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T09:10:18Z","timestamp":1759828218354,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Cervical cancer remains a significant global health concern, primarily associated with persistent infections by high\u2010risk human papillomavirus (hr\u2010HPV). As screening programmes evolve from traditional cytology to DNA\u2010HPV testing, the need for a liquid medium that maintains the integrity of cervical samples for biomolecular analysis and cytology becomes critical.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study evaluated the performance of the candidate liquid preservation medium (PM) Cytoliq for cervical samples intended for DNA\u2010HPV testing and liquid\u2010based cytology (LBC), in comparison with the reference PM, PreservCyt\u2010ThinPrep. A total of 112 women aged 18\u201364\u2009years underwent routine gynaecological examinations, with paired cervical samples preserved in both PM for HPV testing and genotyping (Cobas HPV test), and LBC. The study aimed for a sensitivity greater than 90% in detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+), moderate to high agreement in HPV testing results (Kappa index &gt;\u20090.70) and adequate performance in LBC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The candidate PM exhibited non\u2010inferior performance relative to the reference PM. DNA\u2010HPV testing showed a 94.5% agreement rate (Kappa\u2009=\u20090.88) and a sensitivity of 92.9% for CIN2+ detection. Additionally, the candidate PM performed well in LBC smear production, with no significant differences in cytological diagnoses. The agreement in LBC diagnoses was 94.0% (Kappa\u2009=\u20090.79) with the ThinPrep processor and 91.8% (Kappa\u2009=\u20090.63) with the Cytoliq processor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The Cytoliq PM demonstrated comparable efficacy to the reference for DNA\u2010HPV testing and LBC, supporting its potential as an alternative preservation medium in cervical cancer screening programmes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cyt.13495","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T03:41:52Z","timestamp":1745379712000},"page":"552-557","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical Performance of a Liquid Preservation Medium for Cervicovaginal Samples in <scp>DNA<\/scp>\u2010<scp>HPV<\/scp> Testing and Liquid\u2010Based Cytology for Cervical Cancer Screening"],"prefix":"10.1111","volume":"36","author":[{"given":"Larissa Dias","family":"Assun\u00e7\u00e3o","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Campinas (UNICAMP)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle Garcia","family":"Discacciati","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Campinas (UNICAMP)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andressa Germano","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pathology Federal University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Campinas (UNICAMP)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diama Bhadra","family":"Vale","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Campinas (UNICAMP)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0979-376X","authenticated-orcid":false,"given":"Julio Cesar","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Campinas (UNICAMP)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(22)00501-0"},{"volume-title":"Estimate\/2023 \u2010 Cancer Statistics","year":"2023","key":"e_1_2_11_3_1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr2030640"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(13)62218\u20107"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta23280"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.13174"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2014.11.076"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-20-1336"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471\u20100528.17058"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-71735-2"},{"issue":"376","key":"e_1_2_11_12_1","doi-asserted-by":"crossref","DOI":"10.1136\/bmj-2021-068582","article-title":"National Experience in the First Two Years of Primary Human Papillomavirus (HPV) Cervical Screening in an HPV Vaccinated Population in Australia: Observational Study","volume":"30","author":"Smith M. A.","year":"2022","journal-title":"BMJ"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10120729"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/CEJ.0000000000000701"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22572"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000327859"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22842"},{"key":"e_1_2_11_18_1","unstructured":"CONITEC Comiss\u00e3o Nacional de Incorpora\u00e7\u00e3o de Tecnologias no Sistema Unico de Saude Brasil \u201cPORTARIA SECTICS\u2010MS N\u00b0 3: Decision to Incorporate Molecular Tests for the Detection of Oncogenic HPV Within the Scope of the \u2018Unified Health System\u2019 \u2013 \u2018SUS\u2019 \u201d(2024) https:\/\/www.gov.br\/conitec\/pt\u2010br\/midias\/relatorios\/portaria\/2024\/portaria\u2010sectics\u2010ms\u2010no\u20103\u2010de\u20107\u2010de\u2010marco\u2010de\u20102024\/view."},{"key":"e_1_2_11_19_1","unstructured":"ANVISA \u201cAgencia Nacional de Vigilancia Sanitaria \u201d(2024) Brazil Health Products CytoLiq \u2010 Liquid\u2010Based Fixing Solution Register No. 10039370007 https:\/\/consultas.anvisa.gov.br\/#\/saude\/25351662189202111\/?cnpj=46271011000107&situacaoNotificacaoRegistro=1."},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24010"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-11074-5_1"},{"issue":"30","key":"e_1_2_11_23_1","first-page":"19","article-title":"The Bethesda System for Reporting Cervical Cytology","volume":"19","author":"Pangarkar M. A.","year":"2022","journal-title":"CytoJournal"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.13495","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T08:32:17Z","timestamp":1759825937000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.13495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,23]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/cyt.13495"],"URL":"https:\/\/doi.org\/10.1111\/cyt.13495","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"type":"print","value":"0956-5507"},{"type":"electronic","value":"1365-2303"}],"subject":[],"published":{"date-parts":[[2025,4,23]]},"assertion":[{"value":"2025-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}