{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:08:38Z","timestamp":1780484918085,"version":"3.54.1"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Central nervous system (CNS) involvement in paediatric B\u2010acute lymphoblastic leukaemia (B\u2010ALL) significantly impacts relapse risk and survival. Conventional cytomorphology (CM) of cerebrospinal fluid (CSF) is rapid and specific but has low sensitivity because of limited cellularity and morphological overlap with reactive lymphocytes.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To evaluate the diagnostic utility and resource efficiency of immunocytochemistry (ICC) as an adjunct to CM in detecting leukaemic infiltration of CSF in paediatric B\u2010ALL.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In Cohort 1 (\n n<\/jats:italic>\n \u2009=\u200945), all CSF samples underwent CM and ICC using CD3, CD79a, and TdT. In Cohort 2 (\n n<\/jats:italic>\n \u2009=\u200944), ICC was applied selectively to samples with pleocytosis (>\u20094 cells\/\u03bcL) on the basis of a triage algorithm.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n In Cohort 1, ICC upgraded a subset of \u201cdoubtful\u201d CM cases to definitive leukaemic infiltration, particularly with CD79a and TdT. CD3 reliably confirmed reactive T\u2010cell morphology, enhancing specificity. In Cohort 2, selective ICC use maintained diagnostic yield, detecting blasts in doubtful cases while conserving slides and resources. Together, ICC demonstrated added diagnostic value, especially in morphologically equivocal and higher\u2010cellularity specimens.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n ICC complements CM in CSF evaluation for paediatric B\u2010ALL, improving sensitivity and supporting a pragmatic triage strategy. A combination of CD3 and CD79a is optimal when two smears are available; with limited material, marker choice should be guided by morphology. This cost\u2010effective workflow enhances detection of CNS involvement where flow cytometry or molecular assays are not readily available.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cyt.70060","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:25:38Z","timestamp":1773757538000},"page":"403-409","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of Immunocytochemistry in Cerebrospinal Fluid of B\u2010\n ALL<\/scp>\n Patients for\n CNS<\/scp>\n Involvement: A Pilot Study"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7414-4096","authenticated-orcid":false,"given":"Sherrin","family":"Jacob","sequence":"first","affiliation":[{"name":"Senior Resident, Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lavleen","family":"Singh","sequence":"additional","affiliation":[{"name":"Assistant Professor, Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkateswaran K.","family":"Iyer","sequence":"additional","affiliation":[{"name":"Professor and Head, Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachna","family":"Seth","sequence":"additional","affiliation":[{"name":"Professor, Department of Pediatric Oncology All India Institute of Medical Sciences New Delhi 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