{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:51:08Z","timestamp":1791273068166,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004482","name":"Kuwait University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pancreatic adenocarcinoma (PDAC) arises from transformed pancreatic stem cells. Different stemness pathways are thought to be involved in the progression of PDAC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess the expression of four stem cell markers to determine the best candidate for targeted therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seventy\u2010one PDAC cell blocks were immunoassayed for CD24, OCT4, DCLK1 and CD44 expression and genotyped for common\n                      <jats:italic>KRAS<\/jats:italic>\n                      mutations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      OCT4 was detected in 82.9% of PDAC and undetected in healthy pancreas. CD24 was detected in 23.9% of PDACs, while DCLK1 and CD44 were detected in 25.7% and 34.8% of PDACs, respectively. CD24 localisation was primarily nuclear in PDAC and membranous in healthy pancreas tissues. OCT4 expression and nuclear localisation correlated positively with CD24 expression and nuclear localisation (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.264,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.023;\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.238,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.041, respectively). OCT4 expression was lower in\n                      <jats:italic>KRAS<\/jats:italic>\n                      mutation\u2010positive specimens (\u03b2 \u22120.54, 95% CI: \u22120.37 \u2013 (\u22120.088),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      OCT4 expression is a specific biomarker for PDAC. Its increased expression signified PDAC progression and CD24 activation in a subset of PDAC.\n                      <jats:italic>KRAS<\/jats:italic>\n                      mutants have lower OCT4 expression, suggesting an alternative mechanism for cancer progression than that in OCT4 positive PDAC.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cyt.70072","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T05:21:01Z","timestamp":1774329661000},"page":"609-615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immunocytochemical Analysis of Stem Cell Markers in Pancreatic Adenocarcinoma"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5662-7684","authenticated-orcid":false,"given":"Rabeah","family":"Al\u2010Temaimi","sequence":"first","affiliation":[{"name":"Department of Pathology, College of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Al\u2010Ali","sequence":"additional","affiliation":[{"name":"Department of Medicine College of Medicine, Kuwait University  Jabriya Kuwait"},{"name":"Department of Gastroenterology and Hepatology Mubarak Al Kabeer Hospital  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bency","family":"John","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4559-7608","authenticated-orcid":false,"given":"Kusum","family":"Kapila","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine Kuwait University  Jabriya Kuwait"},{"name":"Department of Laboratory Medicine Mubarak Al Kabeer Hospital  Jabriya 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