{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T12:25:00Z","timestamp":1787142300543,"version":"build-2736575974"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015160","name":"Hologic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015160","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytopathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To analyse the impact of a previous negative mRNA HPV test in the immediate and 6\u2009year risk of developing CIN2+\/CIN3+ lesions in women with low\u2010grade (LG, including ASC\u2010US and LSIL) cytology in the second round of cotest screening.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A prospective longitudinal study with LBC (ThinPrep, Hologic) and mRNA Aptima HPV test. Cotest was performed to 5053 asymptomatic women aged 25\u201365\u2009years within an opportunistic screening programme in Madrid. Women with LG cytology, regardless of the mRNA HPV test result, were followed up (FU) to 10\u2009years. The interventional arm \u2018RNA\u2010based Extended Interventional Nucleic Acid (REINA)\u2019 recruited 2000 women with negative results in the first round for a second cotest 4\u2009years later, and were FU to 6 more years. The risk of a CIN2+\/CIN3+ at 6\u2009years after a first LG cytology was calculated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      With an unknown HPV history, 18.7% of mRNA HPV\u2010positive women at first cotest had LG cytology. Their immediate CIN2+ and CIN3+ risks were 24.7% and 12.8%, respectively, increasing to 31.8% and 17.0% after a 6\u2010year FU. The long\u2010term incidence of CIN2+ was strongly associated with mRNA HPV infection (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.00001). From REINA, 20% of women with a new positive mRNA HPV result at second cotest had LG cytology, carrying much lower immediate and 6\u2010year cumulative CIN2\/CIN3 risks (5.0% and 10.0% respectively).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A previous negative Aptima mRNA HPV test confers a protective effect for CIN2+\/CIN3+ in mRNA HPV\u2010positive women with minor cytological changes. The adjusted risks impact patient management, decreasing colposcopy referrals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cyt.70090","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T03:56:46Z","timestamp":1779767806000},"page":"531-537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RNA Extended Interventional Nucleic Acid (REINA) Longitudinal Study: Decreased Risk of CIN2+\/CIN3+ With Low\u2010Grade Cytology After A Previous Negative HPV Test"],"prefix":"10.1111","volume":"37","author":[{"given":"Rosario","family":"Granados","sequence":"first","affiliation":[{"name":"Department of Pathology Hospital Universitario de Getafe  Madrid Spain"},{"name":"Universidad Europea de Madrid Facultad de Medicina, Salud y Deportes. Departamento de Medicina  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanny A.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Universitario de Getafe  Madrid Spain"},{"name":"Universidad Europea de Madrid Facultad de Medicina, Salud y Deportes. Departamento de Medicina  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Luj\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Universitario de Getafe  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Gutierrez\u2010Pecharrom\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Universitario de Getafe  Madrid Spain"},{"name":"Universidad Europea de Madrid Facultad de Medicina, Salud y Deportes. Departamento de Medicina  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Sol\u00eds","sequence":"additional","affiliation":[{"name":"Department of Gynecology Hospital Universitario de Getafe  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Molpeceres","sequence":"additional","affiliation":[{"name":"Department of Gynecology Hospital Universitario de Getafe  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Bajo","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Universitario de Getafe  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.13174"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr2030640"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000667"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-021-01614-4"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000529"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22895"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21521"},{"key":"e_1_2_13_9_1","unstructured":"AEPCC\u2010Gu\u00eda \u201cPrevenci\u00f3n secundaria del cancer de cuello del \u00fatero 2022. Conducta cl\u00ednica ante resultados anormales de las pruebas de cribado \u201d(2022) Coordinador: Torn\u00e9 A. Secretaria: del Pino M. Autores: Torn\u00e9 A; And\u00eda D; Bruni L et\u00a0al."},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/516784"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.07.015"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24066"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03558-14"},{"issue":"8","key":"e_1_2_13_14_1","article-title":"Cytology Versus HPV Testing for Cervical Cancer Screening in the General Population","volume":"8","author":"Koliopoulos G.","year":"2017","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124460"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090023"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181f74885"}],"container-title":["Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.70090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cyt.70090","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cyt.70090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T11:32:30Z","timestamp":1787139150000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cyt.70090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,25]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/cyt.70090"],"URL":"https:\/\/doi.org\/10.1111\/cyt.70090","archive":["Portico"],"relation":{},"ISSN":["0956-5507","1365-2303"],"issn-type":[{"value":"0956-5507","type":"print"},{"value":"1365-2303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,25]]},"assertion":[{"value":"2026-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}