{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T04:05:23Z","timestamp":1774065923308,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Deutsche Derma Gesell"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The measurement of specific IgE (sIgE) antibodies plays a key role in the diagnosis of honeybee and wasp venom allergy. In recent years, component\u2010resolved diagnosis (CRD) has been introduced, which allows for the measurement of sIgE antibodies against Api m 1, Ves v 1, Ves v 5, and Pol d 5, as well as cross\u2010reactive carbohydrate determinants (CCDs). These tests are intended to help determine the clinical relevance of any given sensitization, especially in patients with dual sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>Specific IgE antibody levels were measured in 143 patients with bee and\/or wasp venom allergy using the extract\u2010based ImmunoCAP<jats:sup>\u00ae<\/jats:sup> allergens i1 and i3 as well as the ImmunoCAP<jats:sup>\u00ae<\/jats:sup> allergen components i208\u2010211 and O214 (Api m 1, Ves v 1, Ves v 5, Pol d 5, CCDs). In patients with dual sensitization, inhibition testing was also performed. In a subgroup of the study population, sIgE to Api m 1, Api m 4, Pol d 5, and Ves v 5 were determined using the ISAC<jats:sup>\u00ae<\/jats:sup> allergy microarray (<jats:italic>n<\/jats:italic> = 44).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sensitivity of Ves v 5 in patients with isolated wasp venom allergy was 78.5 %; in combination with Ves v 1, that figure increased to 92.3 %. The sensitivity of Api m 1 in individuals with isolated bee venom allergy was 25 %. CRD and inhibition testing in individuals with dual sensitization showed divergent results. CRD using the ISAC<jats:sup>\u00ae<\/jats:sup> allergy microarray showed marked differences, especially with regard to Api m 1 and CCDs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Component\u2010resolved tests are a valuable addition to the diagnostic spectrum as long as they are used in combination with established procedures. Apart from Ves v 5, measuring IgE antibodies to Ves v 1 should always be included in the diagnostic workup.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ddg.13240","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T14:27:32Z","timestamp":1494340052000},"page":"507-515","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic precision of component\u2010resolved vs. extract\u2010based in vitro diagnosis of hymenoptera venom allergy: effects on clinical management"],"prefix":"10.1111","volume":"15","author":[{"given":"Florian","family":"Seyfarth","sequence":"first","affiliation":[{"name":"Department of Dermatology Jena University Hospital  Jena Germany"},{"name":"Dermatology Practice PD Dr. med. habil. K. 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