{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T09:44:07Z","timestamp":1694252647185},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T00:00:00Z","timestamp":1568332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Deutsche Derma Gesell"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Due to rising skin cancer incidence rates there is an urgent need for a quick, reliable and cost\u2010effective therapy. Previous studies showed that fresh tumor tissue and margins could be directly examined microscopically with high sensitivity and specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Comparing the accuracy of rapid lump examination (RLE) for the detection of basal cell carcinoma (BCC) during micrographic surgery with formalin\u2010fixed paraffin\u2010embedded hematoxylin and eosin (HE)\u2010stained sections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Materials<\/jats:title><jats:p>382 specimens of 118 excised samples with suspected BCC were examined with RLE and compared with formalin\u2010fixed paraffin\u2010embedded HE\u2010stained sections. For RLE, following a standardized 60 sec staining protocol, the fresh tissue samples were observed directly with a stereomicroscope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For BCC, RLE had a high but insufficient diagnostic accuracy compared to the gold standard of formalin\u2010fixed paraffin\u2010embedded HE\u2010stained sections. The sensitivity was 76\u00a0% (95\u00a0% CI = [66.18\u00a0%; 84.5\u00a0%]) and specificity was 91\u00a0% (95\u00a0% CI = [86.73\u00a0%; 93.75\u00a0%]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>RLE is a fast and simple technique for microscopically controlled surgery (MCS) of basal cell carcinomas that requires training. The experience of the examiner has a major influence on the results. RLE has great potential to speed up the workflow in Mohs surgery but should be improved in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ddg.13937","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T09:03:58Z","timestamp":1568365438000},"page":"1131-1139","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rapid Lump Examination (RLE) \u2013 a bedside 3\u2010dimensional microscopy of tumor specimens"],"prefix":"10.1111","volume":"17","author":[{"given":"Nina","family":"Peters","sequence":"first","affiliation":[{"name":"Department of Dermatology University Medical Center Tuebingen  Tuebingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Schubert","sequence":"additional","affiliation":[{"name":"Artemed Fachklinik M\u00fcnchen  Munich 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