{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T04:41:06Z","timestamp":1692592866456},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T00:00:00Z","timestamp":1652659200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Deutsche Derma Gesell"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:sec><jats:title>Hintergrund und Ziele<\/jats:title><jats:p>Pyoderma gangraenosum ist eine ulzerierende, autoinflammatorische Erkrankung. Es gibt keine eindeutigen histopathologischen Merkmale zur Differenzierung von anderen Ursachen chronischer Wunden wie dem Ulcus cruris venosum. Ziel dieser Studie war es, histopathologische Merkmale von Pyoderma gangraenosum und Unterschiede zu ven\u00f6sen Ulzerationen zu detektieren.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patienten und Methodik<\/jats:title><jats:p>Acht Gewebeproben von Pyoderma gangraenosum, zw\u00f6lf Proben von Ulcus cruris venosum und sechs Proben von gesunder Haut wurden einer immunhistologischen Multi\u2010Antigen\u2010Analyse unterzogen. Das Immuninfiltrat und seine r\u00e4umliche Verteilung wurden anhand von Fluoreszenzbildern mit einer Gewebezytometriesoftware analysiert.<\/jats:p><\/jats:sec><jats:sec><jats:title>Ergebnisse<\/jats:title><jats:p>Die dichte epidermale Pr\u00e4senz von CD45RO<jats:sup>+<\/jats:sup>\u2010T\u2010Ged\u00e4chtnis\u2010Zellen und die Rarefizierung von CD1a<jats:sup>+<\/jats:sup>\u2010Langerhans\u2010Zellen in der Epidermis waren Marker f\u00fcr Pyoderma gangraenosum, welche auch auf eine epidermale Immunreaktion schlie\u00dfen lassen. Dar\u00fcber hinaus konnte dermal eine hohe Anzahl CD11c<jats:sup>+<\/jats:sup>CD68<jats:sup>+<\/jats:sup> pro\u2010inflammatorischer M1\u2010Makrophagen nachgewiesen werden. Diese \u00fcberstieg die Anzahl der in ven\u00f6sen Ulzerationen beobachteten Makrophagen deutlich.<\/jats:p><\/jats:sec><jats:sec><jats:title>Schlussfolgerungen<\/jats:title><jats:p>Die histopathologischen Unterschiede zwischen Pyoderma gangraenosum und Ulcus cruris venosum k\u00f6nnen zur Unterscheidung der beiden Erkrankungen herangezogen werden und somit eine wichtige Hilfe zur schnellen Einleitung einer ad\u00e4quaten Therapie sein. Dar\u00fcber hinaus deuten unsere Daten auf einen antigengesteuerten Prozess in der Epidermis hin, m\u00f6glicherweise unter Beteiligung von CD1a<jats:sup>+<\/jats:sup> Langerhans\u2010Zellen.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ddg.14708_g","type":"journal-article","created":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T04:54:40Z","timestamp":1652763280000},"page":"619-628","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epidermotropie von Immunzellen unterscheidet Pyoderma gangraenosum vom Ulcus cruris venosum"],"prefix":"10.1111","volume":"20","author":[{"given":"Moritz","family":"Ronicke","sequence":"first","affiliation":[{"name":"Hautklinik Universit\u00e4tsklinikum Erlangen"},{"name":"Deutsches Zentrum f\u00fcr Immuntherapie (DZI) FAU Erlangen\u2010N\u00fcrnberg  Erlangen"},{"name":"Friedrich\u2010Alexander\u2010Universit\u00e4t Erlangen\u2010N\u00fcrnberg (FAU)  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