{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:21Z","timestamp":1790718681354,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Deutsche Derma Gesell"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background and objectives<\/jats:title><jats:p>Onychomycosis (OM) and tinea pedis (TP) are common fungal infections. Currently, diagnosis is based on direct microscopy and culture that have a low to moderate sensitivity and\/or require up to 3\u20134 weeks until results are obtained. PCR techniques have emerged for the diagnosis of fungal infections, but little is known about their sensitivity and specificity in diagnosing. Here, we compared the diagnostic value of a DNA\u2010chip technology, that detects 56 fungal pathogens, in a single\u2010center prospective diagnostic study with microscopy and culture in suspected OM\/TP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>Microscopy, culture and DNA microarray assays were performed on scraping material from patients with suspected OM (n = 67) or TP (n = 73). To test whether swabs can be used as an alternative for scraping, PCR yields were compared in a further 13 patients with OM and 11 patients with TP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DNA microarrays had the highest sensitivity. Combination of DNA\u2010chip technology with microscopy further increased the sensitivity, and results from this combined laboratory diagnosis can be obtained within 24 hours. Comparison of sampling techniques (scraping, dry or wet swab) for DNA\u2010chip assays showed similar results in suspected OM or TP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>DNA\u2010chip technology\u00a0shows high sensitivity for OM and TP diagnosis, especially when combined with microscopy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ddg.14819","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T21:08:37Z","timestamp":1658264917000},"page":"1112-1121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["DNA chip\u2010based diagnosis of onychomycosis and tinea pedis"],"prefix":"10.1111","volume":"20","author":[{"given":"Katja","family":"Bieber","sequence":"first","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  L\u00fcbeck 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