{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:37:24Z","timestamp":1781109444643,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Deutsche Derma Gesell"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Junctional epidermolysis bullosa (JEB) is a subtype of epidermolysis bullosa caused by mutations in the <jats:italic>LAMB3<\/jats:italic> gene. We treated a patient with JEB using genetically corrected autologous epidermal cultures retrovirally transduced with the functional <jats:italic>LAMB3<\/jats:italic> gene sequence. The objective of this study was to analyze the skin microbiome of this patient, with a particular focus on transgenic skin, and to compare the findings to the skin microbiome of healthy controls and patients with atopic dermatitis and well\u2010documented microbial dysbiosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p>Skin microbiome analysis was performed on a JEB patient 72\u00a0months after combined gene and stem cell therapy. Skin swabs from age\u2010matched healthy controls and atopic dermatitis patients were included from the ProRaD study of CK\u2010CARE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The transgenic skin had comparably high relative and absolute <jats:italic>Staphylococcus (S.) aureus<\/jats:italic> abundance to blistering and non\u2010blistering skin of the JEB patient, while the total bacterial load was lower. In blistering skin of the JEB patient, higher bacterial load was driven by <jats:italic>S. aureus<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our investigation confirms a unique microbiome composition in JEB, characterized by <jats:italic>S. aureus<\/jats:italic> driven bacterial overgrowth. The dysbiosis was not reversed in transgenic, non\u2010blistering skin areas. 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