{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:47:57Z","timestamp":1787014077400,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012910","name":"National Defense Medical College","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012910","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Digestive Endoscopy"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Paneth cell metaplasia (PCM), a metaplastic change associated with chronic inflammation in ulcerative colitis (UC), may be linked to UC\u2010associated neoplasia (UCAN). However, no endoscopic method currently exists for detecting PCM. This study aimed to develop and validate a novel endoscopic staining technique\u2014CV\u2010SCAN\u2014for identifying PCM and UCAN, and to explore the molecular characteristics of the stained areas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective observational study included 131 patients with UC undergoing surveillance colonoscopy. CV\u2010SCAN involved spraying an ultra\u2010diluted solution (0.006%) of crystal violet from the descending colon to the rectum. Biopsies were obtained from stained and non\u2010stained areas and evaluated histologically and molecularly. RNA expression profiles were analyzed via microarray and real\u2010time RT\u2010PCR. The diagnostic performance of CV\u2010SCAN for detecting PCM was assessed, along with its correlation with UCAN history.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CV\u2010SCAN visualized sharply demarcated, purple\u2010stained areas corresponding to PCM or UCAN. PCM was significantly associated with a history of UCAN. Uniform, dark staining was characteristic of PCM, while UCAN showed heterogeneous staining with small round pits. CV\u2010SCAN achieved a sensitivity of 81.3% and a specificity of 84.9% for PCM detection. Molecular analysis revealed upregulation of Paneth cell\u2013specific (DEFA5, DEFA6), small intestinal (CCL25, APOC3), and UCAN\u2010associated (IL17RC) genes, along with downregulation of SATB2 in stained areas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CV\u2010SCAN is a novel and effective endoscopic staining method for detecting PCM and UCAN in patients with UC. It enables risk stratification through direct visualization of precancerous changes and may facilitate early detection and targeted surveillance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/den.70096","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:38:13Z","timestamp":1767775093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CV<\/scp>\n                    \u2010\n                    <scp>SCAN<\/scp>\n                    (Crystal Violet Staining for Colitis\u2010Associated Neoplasia): A Novel Endoscopic Staining Method to Detect Paneth Cell Metaplasia and Ulcerative Colitis (\n                    <scp>UC<\/scp>\n                    )\u2010Associated Neoplasia in\n                    <scp>UC<\/scp>"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7978-0502","authenticated-orcid":false,"given":"Akira","family":"Tomioka","sequence":"first","affiliation":[{"name":"National Defense Medical College Hospital  Tokorozawa\u2010Shi Saitama\u2010Ken Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanoka","family":"Chiya","sequence":"additional","affiliation":[{"name":"National Defense Medical College Hospital  Tokorozawa\u2010Shi Saitama\u2010Ken Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Kurihara","sequence":"additional","affiliation":[{"name":"National Defense Medical College Hospital  Tokorozawa\u2010Shi Saitama\u2010Ken Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikiyo","family":"Okada","sequence":"additional","affiliation":[{"name":"National Defense Medical College Hospital  Tokorozawa\u2010Shi Saitama\u2010Ken 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