{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T10:01:31Z","timestamp":1697536891653},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,10,19]],"date-time":"2012-10-19T00:00:00Z","timestamp":1350604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dev Growth Differ"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Growth factors and their receptors regulate development of many organs through activation of multiple intracellular signaling cascades including a mitogen\u2010activated protein kinase (<jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>). Extracellular regulated kinases (<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>)1\/2, classic <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> family members, are expressed in fetal mouse submandibular glands (<jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>), and stimulate branching morphogenesis. <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5, also called big mitogen\u2010activated protein kinase 1, was recently found as a new member of <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> super family, and its biological roles are still largely unknown. In this study, we investigated the expression and function of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 in developing fetal mouse <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>s. Western blotting analysis showed that the expression pattern of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 was different from the pattern of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 in developing fetal <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>s. Both <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 and <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 were phosphorylated after exposure to ligands of the ErbB family of receptor tyrosine kinases (<jats:styled-content style=\"fixed-case\">RTK<\/jats:styled-content>s). Phosphorylation of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 was strongly induced by epidermal growth factor (<jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>) in <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content> rudiments at embryonic day 14 (<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>14), <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>16 and <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>18. However, <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 phosphorylation induced by <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> was clearly observed at <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>14 and <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>16, but not at <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>18. Branching morphogenesis of cultured <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>13 <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content> rudiments was strongly suppressed by administration of <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>0126, an inhibitor for <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 activation, whereas the phosphorylation of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 was not inhibited by <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>0126. <jats:styled-content style=\"fixed-case\">BIX<\/jats:styled-content>02188, a specific inhibitor for <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 activation, also inhibited branching morphogenesis in cultured <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content> rudiments. These results show that <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\u2010responsive <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 is expressed in developing fetal mouse <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>, and suggest that both <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 and <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>5 signaling cascades might play an important role in the regulation of branching morphogenesis.<\/jats:p>","DOI":"10.1111\/dgd.12008","type":"journal-article","created":{"date-parts":[[2012,10,19]],"date-time":"2012-10-19T10:27:36Z","timestamp":1350642456000},"page":"801-808","source":"Crossref","is-referenced-by-count":6,"title":["Extracellular regulated kinase5 is expressed in fetal mouse submandibular glands and is phosphorylated in response to epidermal growth factor and other ligands of the <scp>E<\/scp>rb<scp>B<\/scp> family of receptors"],"prefix":"10.1111","volume":"54","author":[{"given":"Noriko","family":"Koyama","sequence":"first","affiliation":[{"name":"Department of Pharmacology Asahi University School of Dentistry Hozumi Mizuho Gifu 501\u20100296 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Asahi University School of Dentistry Hozumi Mizuho Gifu 501\u20100296 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Mizukoshi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Asahi University School of Dentistry Hozumi Mizuho Gifu 501\u20100296 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuguteru","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Asahi University School of Dentistry Hozumi Mizuho Gifu 501\u20100296 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward W.","family":"Gresik","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy The City University of New York, The Sophie Davis School of Biomedical Education 160 Convent Ave New York NY 10031 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Kashimata","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Asahi University School of Dentistry Hozumi Mizuho Gifu 501\u20100296 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.28.16586"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.46.27489"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.51.36035"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(98)00522-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(98)00027-6"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)60336-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-999-0014-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012289631-6\/50031-4"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2152\/jmi.56.228"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80562-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10377"},{"key":"e_1_2_6_13_1","first-page":"156","volume-title":"Branching Morphogenesis","author":"Jaskoll T.","year":"2004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26563"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199702)208:2<149::AID-AJA2>3.0.CO;2-I"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9639"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.23.7054"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10309"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2008.01053.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2189"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2011.01.006"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60765-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.03.051"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110020401"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20078"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02651-5"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C300313200"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.112.3.855"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2008.02.016"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.027821"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3409"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2006.00088.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.38.24301"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2011.04.003"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.04.031"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0206OC"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.142293999"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2007.08.010"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20909"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1994.059.01.021"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)80026-X"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207573200"},{"key":"e_1_2_6_43_1","first-page":"223","volume-title":"Developmental Biology, A Comprehensive Synthesis. Vol. 3. The Cell Surface in Development and Cancer","author":"Spooner B. S.","year":"1986"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01690"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003719200"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.09.087"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2007.01.006"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90801-K"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0351"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710308200"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-3-11"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-4023"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313465200"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.21.12665"}],"container-title":["Development, Growth &amp; Differentiation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fdgd.12008","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/dgd.12008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T04:02:47Z","timestamp":1697428967000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/dgd.12008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,19]]},"references-count":53,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/dgd.12008"],"URL":"https:\/\/doi.org\/10.1111\/dgd.12008","archive":["Portico"],"relation":{},"ISSN":["0012-1592","1440-169X"],"issn-type":[{"value":"0012-1592","type":"print"},{"value":"1440-169X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,19]]}}}