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It has been suggested that Greatwall (GWL) kinase was crucial for the M\u2010phase entry and could maintain cyclin B\u2013Cdc2 activity through regulation of protein phosphatase 2A (PP2A), a counteracting phosphatase of MPF. Here, the role of GWL was assessed during release of mouse oocytes from prophase I arrest. GWL was crucial for meiotic maturation in mouse oocytes. As a positive regulator for meiosis resumption, GWL was continually expressed in germinal vesicle (GV) and MII stage oocytes and two\u2010cell stage embryos. Additionally, GWL localized to the nucleus and dispersed into cytoplasm during GV breakdown (GVBD). Furthermore, downregulation of GWL or overexpression of catalytically\u2010inactive GWL inhibited partial meiotic maturation. This prophase I arrest induced by GWL depletion could be rescued by the PP2A inhibition. However, both GWL\u2010depleted and rescued oocytes had severe spindle defects that hardly reached MII. In contrast, oocytes overexpressing wild\u2010type GWL resumed meiosis and progressed to MII stage. Thus, our data demonstrate that GWL acts in a pathway with PP2A which is essential for prophase I exit and metaphase I microtubule assembly in mouse oocytes.<\/jats:p>","DOI":"10.1111\/dgd.12183","type":"journal-article","created":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T02:10:52Z","timestamp":1417745452000},"page":"669-678","source":"Crossref","is-referenced-by-count":9,"title":["Role of Greatwall kinase in release of mouse oocytes from diplotene arrest"],"prefix":"10.1111","volume":"56","author":[{"given":"Xiangyu","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Biochemical and Molecular Biology China Medical University Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dahai","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Molecular Biology China Medical University Shenyang Liaoning Province 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