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The markers used in these analyses included <jats:italic>pdx1<\/jats:italic>,<jats:italic> nkx6.1<\/jats:italic>,<jats:italic> nkx6.2<\/jats:italic>,<jats:italic> nkx2.2<\/jats:italic>, Islet1, <jats:italic>insulin<\/jats:italic>, Somatostatin, <jats:italic>glucagon<\/jats:italic>,<jats:italic> ptf1a<\/jats:italic>,<jats:italic> ela3l<\/jats:italic>,<jats:italic> trypsin<\/jats:italic>, and <jats:italic>amylase<\/jats:italic>. The double transgenic (Tg) fish produced in the present study visualizes the development of endocrine (<jats:italic>pdx1<\/jats:italic>+) and exocrine (<jats:italic>ptf1a<\/jats:italic>+) parts simultaneously in living fishes. Like other vertebrates, the medaka pancreas develops as two (dorsal and ventral) buds in the anterior gut tube, which soon fuse into a single anlagen. The double Tg fish demonstrates that the differential property between the two buds is already established at the initial phase of bud development as indicated by strong <jats:italic>pdx1<\/jats:italic> expression in the dorsal one. This Tg fish also allowed us to examine the gross morphology and the structure of adult pancreas and revealed unique characters of medaka pancreas such as broad and multiple connections with the gut tube along the anterior\u2013posterior axis.<\/jats:p>","DOI":"10.1111\/dgd.12237","type":"journal-article","created":{"date-parts":[[2015,10,5]],"date-time":"2015-10-05T08:29:14Z","timestamp":1444033754000},"page":"557-569","source":"Crossref","is-referenced-by-count":9,"title":["Development of the pancreas in medaka, <i><scp>O<\/scp>ryzias latipes<\/i>, from embryo to adult"],"prefix":"10.1111","volume":"57","author":[{"given":"Takayoshi","family":"Otsuka","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Graduate School of Science University of Tokyo Hongo Bunkyo\u2010ku Tokyo 113\u20100033 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Tsukahara","sequence":"additional","affiliation":[{"name":"Department of Biological 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